Thermo Fisher Scientific CHOP Monoclonal Antibody (9C8)

Applications

Product Specifications

Product Specific Information

MA1-250 detects CHOP protein in human and mouse samples.
It has been successfully used in Western blot, immunoprecipitation, immunocytochemistry, and immunofluorescence procedures.
By Western blot, this antibody detects an ~31 kDa CHOP protein from primary human fibroblast extract.

The immunizing protein corresponds to a bacterially expressed, mouse CHOP fusion protein.
Clone 9C8 recognizes an epitope in the N-terminal region of CHOP.
Originally validated as part of a Thermo Scientific Cellomics High Content Screening Kit.
The antibody sold separately may require further optimization for best results.

Target Information

GADD153 (also known as CHOP) is a small nuclear protein capable of dimerizing with transcription factors C/EBP alpha and beta.
This complex inhibits normal C/EBP binding to classical sites and activates binding to non-classical, stress-related targets.
Under normal conditions, GADD153 expression is low but upregulated under cellular or ER stress, such as tunicamycin treatment, nutrient starvation, or reducing agents affecting ER calcium flux.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.