Thermo Fisher Scientific CHOP Monoclonal Antibody (9C8)

Applications

Tested Dilution

Publications

Western Blot (WB)

1 µg/mL

View 73 publications 73 publications

Immunohistochemistry (IHC)

-

View 9 publications 9 publications

Immunohistochemistry (Paraffin) (IHC (P))

Assay-dependent

View 1 publication 1 publication

Immunohistochemistry (Frozen) (IHC (F))

1/100

-

Immunocytochemistry (ICC/IF)

Assay-dependent

View 9 publications 9 publications

Immunoprecipitation (IP)

Assay-dependent

-

Miscellaneous PubMed (Misc)

-

View 3 publications 3 publications

Product Specifications

Species Reactivity

Human, Mouse

Published species

Human, Mouse, Rabbit, Rat, Sheep

Host/Isotype

Mouse / IgG2b

Class

Monoclonal

Type

Antibody

Clone

9C8

Immunogen

A bacterially expressed, mouse CHOP fusion protein.

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Protein A

Storage buffer

PBS with 1mg/mL BSA

Contains

0.05% sodium azide

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2292611

Product Specific Information

MA1-250 detects CHOP protein in human and mouse samples.

MA1-250 has successfully been used in Western blot, immunoprecipitation, immunocytochemistry, and immunofluorescence procedures. By Western blot, this antibody detects an ~31 kDa protein representing CHOP from primary human fibroblast extract.

The MA1-250 immunizing protein corresponds to a bacterially expressed, mouse CHOP fusion protein. Clone 9C8 has been shown to recognize an epitope in the N-terminal region of CHOP.

This antibody was orginally validated as part of a Thermo Scientific Cellomics High Content Screening Kit. The antibody sold separately may have slightly different performance and may need to be further optimized for the best results.

Target Information

GADD153 is a small nuclear protein that is capable of dimerizing with transcription factors C/EBP alpha and beta. Once dimerized, this complex inhibits the normal binding and function of C/EBP to classical binding sites. Inversely, the C/EBP GADD153 dimer gains binding activity to other non classical C/EBP stress related targets. Under normal cellular conditions this protein is not expressed in detectable levels, but is highly unregulated during times of cellular/ER stress. Examples of GADD153 inducing stress include: treatment with tunicamycin, nutrient starvation and reducing agents that interfere with the calcium flux across the ER membrane.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.