Thermo Fisher Scientific S100B Monoclonal Antibody (SH-B4)
S100B 단백질을 인식하는 SH-B4 클론의 mouse monoclonal antibody. Western blot, IHC, ICC 등 다양한 응용에 사용 가능. Human 등 여러 종에 반응하며, 11.5 mg/mL 농도의 액상 형태로 제공. 4°C 단기 보관, -20°C 장기 보관 권장.
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Applications and Tested Dilutions
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 0.1 µg/mL | - |
| Immunohistochemistry (IHC) | - | 3 publications |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:100 | - |
| Immunohistochemistry (Frozen) (IHC (F)) | Assay-dependent | - |
| Immunocytochemistry (ICC/IF) | Assay-dependent | 2 publications |
| ELISA | Assay-dependent | - |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Bovine, Dog, Cat, Goat, Human, Sheep, Pig, Rabbit, Rat |
| Published Species | Human, Mouse |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | SH-B4 |
| Immunogen | Purified, full length, native bovine S100B protein |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 11.5 mg/mL |
| Storage Buffer | Ascites |
| Contains | 15 mM sodium azide |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_795382 |
Product Specific Information
- Recommended positive control: human tongue
- Predicted molecular weight: 10.5 kDa
- Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
Target Information
S100B is a member of the S100 family of proteins containing two EF-hand calcium-binding motifs. S100 proteins are localized in the cytoplasm and/or nucleus of a wide range of cells and play roles in regulating processes such as cell cycle progression and differentiation.
S100B may function in neurite extension, proliferation of melanoma cells, stimulation of Ca²⁺ fluxes, inhibition of PKC-mediated phosphorylation, astrocytosis and axonal proliferation, and inhibition of microtubule assembly.
Chromosomal rearrangements and altered expression of this gene have been implicated in diseases including Alzheimer’s disease, Down’s syndrome, epilepsy, amyotrophic lateral sclerosis, melanoma, and type I diabetes.
For Research Use Only.
Not for use in diagnostic procedures. Not for resale without express authorization.
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