Thermo Fisher Scientific Arginase 1 Monoclonal Antibody (A1exF5), PerCP-eFluor 710, eBioscience
인간 및 마우스 Arginase 1을 인식하는 단클론 항체로, PerCP-eFluor 710 형광 표지로 Flow Cytometry에 최적화됨. 488 nm 청색 레이저로 여기, 710 nm에서 방출. 세포 내 염색 및 Foxp3 분석에 적합하며, 빛에 민감하므로 암조건 보관 필요.
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Thermo Fisher Scientific Arginase 1 Monoclonal Antibody (A1exF5), PerCP-eFluor 710, eBioscience
Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Immunohistochemistry (IHC) | - | View 1 publication |
| Flow Cytometry (Flow) | 0.25 µg/test | View 23 publications |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse |
| Published Species | Human, Mouse |
| Host / Isotype | Rat / IgG2a, kappa |
| Recommended Isotype Control | Rat IgG2a kappa Isotype Control (eBR2a), PerCP-eFluor™ 710 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | A1exF5 |
| Immunogen | E.coli-derived Recombinant mouse Arginase 1 |
| Conjugate | PerCP-eFluor™ 710 |
| Excitation / Emission Max | 482 / 708 nm |
| Form | Liquid |
| Concentration | 0.2 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2 |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C, store in dark, DO NOT FREEZE |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2734842 |
Additional Formats Available
- Alexa Fluor 488
- Alexa Fluor 700
- APC
- Brilliant UV 805
- Brilliant Violet 421
- Brilliant Violet 480
- Brilliant Violet 650
- Brilliant Violet 711
- eFluor 450
- PE
- PE-Cyanine7
- Request custom conjugation
Product Specific Information
The monoclonal antibody A1exF5 recognizes both human and mouse Arginase 1 (Arg1), a cytosolic enzyme.
This clone is compatible with standard intracellular staining and Foxp3/Transcription Factor Staining Buffer Set protocols.
Applications Reported: Flow cytometric analysis
Applications Tested: Tested on stimulated mouse bone marrow cells using the Intracellular Fixation & Permeabilization Buffer Set (Product #88-8824-00).
Recommended usage: ≤0.25 µg per test (100 µL final volume, 10⁵–10⁸ cells/test). Optimal titration is recommended.
Fluorophore Information:
PerCP-eFluor™ 710 emits at 710 nm and is excited at 488 nm (blue laser).
Recommended filter: 710/50 bandpass (695/40 acceptable alternative).
Light sensitive – protect from light.
Fixation Guidance:
Samples can be stored in IC Fixation Buffer (Product #00-8222) or 1-step Fix/Lyse Solution (Product #00-5333) for up to 3 days at 4°C in dark conditions with minimal impact on brightness or FRET efficiency.
Excitation: 488 nm
Emission: 710 nm
Laser: Blue Laser
Target Information
Arginase-1 (Arg1) is a 35 kDa enzyme that converts L-arginine to urea and L-ornithine in the final step of the urea cycle.
It plays a role in cell proliferation and detoxification by regulating nitric oxide production through arginine degradation.
Expression:
- Human: liver, neutrophils, myeloid-derived suppressor cells (MDSC), neural stem cells
- Mouse: liver, alternatively activated macrophages (M2a), tumor-infiltrating myeloid cells
Defects in Arginase 1 cause argininemia, an autosomal recessive disorder characterized by hyperammonemia.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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