Thermo Fisher Scientific Kir2.1 (KCNJ2) Monoclonal Antibody (S112), FITC
Kir2.1(KCNJ2) 단백질을 인식하는 FITC 결합 단일클론 항체로, Western blot, IHC, ICC/IF 등 다양한 분석에 적합합니다. 사람, 마우스, 랫드 등에서 반응하며, 단백질 G로 정제된 고순도 항체입니다. 4°C 암소 보관 권장.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:1,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:1,000 |
| Immunocytochemistry (ICC/IF) | 1:100 |
| Antibody Microarray (AM) | Assay-dependent |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse, Non-human primate, Rat |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | S112 |
| Immunogen | Fusion protein amino acids 41–64 and 189–428 of mouse Kir2.1 |
| Conjugate | FITC |
| Excitation / Emission Max | 498 / 517 nm |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein G |
| Storage Buffer | 9.1 mM sodium bicarbonate/PBS, pH 7.4, with 640.91 mM DMSO, 136.36 mM ethanolamine |
| Contains | No preservative |
| Storage Conditions | 4°C, store in dark |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2931870 |
Available Formats
- Unconjugated (MA5-27681)
- APC (MA5-45415)
- PE (MA5-45418)
- PerCP (MA5-45417)
- Custom conjugation available
Product Specific Information
1 µg/mL of MA5-45416 was sufficient for detection of Kir2.1 in 10 µg of rat brain lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody. Detects approximately 45 kDa. No cross-reactivity against Kir2.2 or Kir2.3.
Target Information
The KIR2 subunit family includes 2.1, 2.2, 2.3, and 2.4. Unlike G protein-coupled KIR3 subunits, KIR2.1 requires both phosphorylation by PKA and ATP hydrolysis for functional activity. KIR2.1 is expressed in the superior and inferior colliculi and the pontine region of the brain, moderating synaptic transmission. In the placenta, KIR2.1 is expressed throughout gestation in cytotrophoblast cells. In the kidney, KIR2.1 colocalizes with KIR5.1 in the proximal tubule. KIR2.1, 2.2, and 2.3 associate with the membrane-associated guanylate kinase SAP97 in the cerebellum and heart. Phosphorylation of KIR2.2 by protein kinase A inhibits its association with SAP97.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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