Thermo Fisher Scientific Kir2.1 (KCNJ2) Monoclonal Antibody (S112), PE
Kir2.1 (KCNJ2) 단백질을 검출하기 위한 Thermo Fisher Scientific의 PE 결합 단일클론 항체입니다. Western blot, IHC, ICC/IF 등 다양한 응용에 적합하며, 인간, 마우스, 설치류 등에서 반응합니다. 고순도 Protein G 정제, 액상 형태로 제공됩니다.
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Thermo Fisher Scientific Kir2.1 (KCNJ2) Monoclonal Antibody (S112), PE
Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:1,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:1,000 |
| Immunocytochemistry (ICC/IF) | 1:100 |
| Antibody Microarray (AM) | Assay-dependent |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse, Non-human primate, Rat |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | S112 |
| Immunogen | Fusion protein amino acids 41–64 and 189–428 of mouse Kir2.1 |
| Conjugate | PE |
| Excitation / Emission Max | 565 / 576 nm |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein G |
| Storage Buffer | 95.64 mM phosphate / 2.48 mM MES, pH 7.4, with 0.5 M EDTA |
| Contains | No preservative |
| Storage Conditions | 4°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2931872 |
Additional Formats
- Unconjugated: MA5-27681
- APC: MA5-45415
- FITC: MA5-45416
- PerCP: MA5-45417
- Request custom conjugation available
Product Specific Information
1 µg/mL of MA5-45418 was sufficient for detection of Kir2.1 in 10 µg of rat brain lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.
Detects approximately 45 kDa. No cross-reactivity against Kir2.2 or Kir2.3.
Target Information
The KIR2 subunit family includes 2.1, 2.2, 2.3, and 2.4. Unlike G protein-coupled KIR3 subunits, KIR2.1 requires phosphorylation by PKA and ATP hydrolysis for functional activity.
KIR2.1 is expressed in the superior and inferior colliculi and the pontine region of the brain, moderating synaptic transmission. In the placenta, it is expressed throughout gestation in cytotrophoblast cells. In the kidney, KIR2.1 colocalizes with KIR5.1 in the proximal tubule.
KIR2.1, 2.2, and 2.3 associate with the membrane-associated guanylate kinase synapse-associated protein 97 (SAP97) in the cerebellum and heart. Phosphorylation of KIR2.2 by protein kinase A inhibits association with SAP97.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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