Thermo Fisher Scientific Kir2.1 (KCNJ2) Monoclonal Antibody (S112), APC
Kir2.1(KCNJ2) 단백질을 인식하는 Mouse Monoclonal Antibody로 APC 형광표지. Western blot, IHC, ICC/IF 등 다양한 응용에 적합. Human, Mouse, Rat 등 반응성 보유. 단백질 G 정제, 보존제 무첨가, 4°C 보관.
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Thermo Fisher Scientific Kir2.1 (KCNJ2) Monoclonal Antibody (S112), APC
Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:1,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:1,000 |
| Immunocytochemistry (ICC/IF) | 1:100 |
| Antibody Microarray (AM) | Assay-dependent |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human, Mouse, Non-human primate, Rat |
| Host/Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | S112 |
| Immunogen | Fusion protein amino acids 41–64 and 189–428 of mouse Kir2.1 |
| Conjugate | APC |
| Excitation/Emission Max | 651/660 nm |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein G |
| Storage buffer | 95.64 mM phosphate / 2.48 mM MES, pH 7.4, with 0.5 M EDTA |
| Contains | No preservative |
| Storage conditions | 4°C |
| Shipping conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2931869 |
Available Formats
- Unconjugated (MA5-27681)
- FITC (MA5-45416)
- PE (MA5-45418)
- PerCP (MA5-45417)
- Custom conjugation available upon request
Product Specific Information
1 µg/mL of MA5-45415 was sufficient for detection of Kir2.1 in 10 µg of rat brain lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.
Detects approximately 45 kDa. No cross-reactivity against Kir2.2 or Kir2.3.
Target Information
The KIR2 subunit family includes 2.1, 2.2, 2.3, and 2.4.
Unlike G protein-coupled KIR3 subunits, KIR2.1 requires both phosphorylation by PKA and ATP hydrolysis for functional activity.
KIR2.1 is expressed in the superior and inferior collicula and the pontine region of the brain, moderating synaptic transmission.
In the placenta, KIR2.1 is expressed throughout gestation in cytotrophoblast cells.
In the kidney, KIR2.1 colocalizes with KIR5.1 in the proximal tubule.
KIR2.1, 2.2, and 2.3 associate with the membrane-associated guanylate kinase synapse-associated protein 97 in the cerebellum and heart.
Phosphorylation of KIR2.2 by protein kinase A inhibits its association with SAP97.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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