Thermo Fisher Scientific IL-17A Monoclonal Antibody (eBio64DEC17), eFluor 660, eBioscience
인간 IL-17A를 인식하는 중화 단일클론 항체로, eFluor 660 형광 표지 적용. Flow cytometry 및 intracellular staining에 최적화되어 있으며, 염증성 질환 연구에 유용. 4°C 암소 보관, 동결 금지.
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Thermo Fisher Scientific IL-17A Monoclonal Antibody (eBio64DEC17), eFluor 660
Applications and Tested Dilutions
| Application | Tested Dilution | Publications |
|---|---|---|
| Immunohistochemistry (IHC) | - | View 3 publications |
| Immunohistochemistry (Paraffin) (IHC (P)) | - | View 1 publication |
| Immunocytochemistry (ICC/IF) | - | View 3 publications |
| Flow Cytometry (Flow) | 5 µL (0.03 µg)/test | View 27 publications |
| ELISA | - | View 4 publications |
| Neutralization | - | View 1 publication |
| Radioimmune Assays (RIA) | - | View 1 publication |
Product Specifications
| Specification | Detail |
|---|---|
| Published Species | Hamster, Human |
| Host / Isotype | Mouse / IgG1, kappa |
| Recommended Isotype Control | Mouse IgG1 kappa Isotype Control (P3.6.2.8.1), eFluor™ 660 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | eBio64DEC17 |
| Conjugate | eFluor™ 660 |
| Excitation / Emission Max | 651 / 668 nm |
| Form | Liquid |
| Concentration | 5 µL/Test |
| Storage Conditions | 4°C, store in dark, DO NOT FREEZE |
| Shipping Conditions | Wet ice |
| RRID | AB_11150251 |
Available Formats
- Unconjugated
- Alexa Fluor 488
- APC
- APC-eFluor 780
- Biotin
- Brilliant UV 563 / 737 / 805
- Brilliant Violet 605 / 786
- eFluor 450 / 506
- FITC
- PE / PE-Cyanine7 / PE-eFluor 610
- PerCP-Cyanine5.5
- Request custom conjugation
Product Specific Information
The eBio64DEC17 antibody reacts with human IL-17A and functions as a neutralizing antibody. IL-17A is a CD4+ T cell-derived cytokine that promotes inflammatory responses and is elevated in rheumatoid arthritis, asthma, multiple sclerosis, psoriasis, and transplant rejection.
Human IL-17A exists as glycosylated 20–30 kDa homodimers produced by activated CD4+ T-cells. It enhances ICAM-1 expression in fibroblasts and stimulates secretion of IL-6, IL-8, G-CSF, and PGE2 from epithelial and endothelial cells. Fibroblasts exposed to IL-17A support CD34+ progenitor proliferation and neutrophil maturation.
IL-23-dependent Th17 cells produce IL-17A, distinct from Th1/Th2 pathways. IFNγ and IL-4 negatively regulate Th17 cell generation. Intracellular staining by eBio64DEC17 identifies the same population as eBio64CAP17.
Applications Reported: Intracellular staining followed by flow cytometric analysis.
Applications Tested: Pre-titrated for intracellular staining and flow cytometry of stimulated human peripheral blood cells. Use at 5 µL (0.03 µg) per test (100 µL final volume, 10⁵–10⁸ cells/test).
Fluorophore Information:
eFluor® 660 replaces Alexa Fluor® 647. Emission at 659 nm, excitation with red laser (633 nm).
Excitation: 633–647 nm; Emission: 668 nm; Laser: Red.
Filtration: 0.2 µm post-manufacturing filtered.
Target Information
Interleukin-17A (IL-17A, CTLA-8) is a 32 kDa disulfide-linked homodimer (136 amino acids) that signals through IL-17R/CDw217. It regulates NF-κB and MAPK pathways, enhancing IL-6 and COX-2 expression and nitric oxide production. IL-17A family includes IL-17A–F proteins. High IL-17A levels are linked to inflammatory and autoimmune conditions.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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