Thermo Fisher Scientific IL-21 Monoclonal Antibody (eBio3A3-N2 (3A3-N2)), eFluor 660, eBioscience
인간 IL-21을 인식하는 단클론 항체로, NK 및 T세포 활성 연구에 적합. eFluor 660 형광 표지로 Flow Cytometry에 최적화. Th17 세포 내 염색용으로 검증됨. Alexa Fluor 647 대체 형광체 사용.
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Thermo Fisher Scientific IL-21 Monoclonal Antibody (eBio3A3-N2 (3A3-N2)), eFluor 660
Applications
- Flow Cytometry (Intracellular staining)
Tested Dilution: 5 µL (0.125 µg)/test
Publications: View 8 publications
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Published Species | Human |
| Host / Isotype | Mouse / IgG1, kappa |
| Recommended Isotype Control | Mouse IgG1 kappa Isotype Control (P3.6.2.8.1), eFluor™ 660, eBioscience™ |
| Class | Monoclonal |
| Type | Antibody |
| Clone | eBio3A3-N2 (3A3-N2) |
| Conjugate | eFluor™ 660 |
| Excitation/Emission Max | 651 / 668 nm |
| Form | Liquid |
| Concentration | 5 µL/test |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2, with 0.2% BSA |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C, store in dark, DO NOT FREEZE |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_10596354 |
Product Specific Information
Description:
Monoclonal antibody eBio3A3-N2 reacts with human IL-21, a potent stimulator of NK and cytolytic T cells. IL-21 (131 amino acids) is closely related to IL-2 and IL-15 and is expressed in activated CD4+ cells. It stimulates B-cell proliferation via CD40 antigen crosslinking, bone marrow progenitor proliferation, and NK-cell marker CD56 expression in presence of IL-15.
Applications Reported:
Validated for intracellular staining and flow cytometric analysis.
Applications Tested:
Pre-titrated and tested for intracellular staining of restimulated, Th17-polarized human peripheral blood cells. Recommended use: 5 µL (0.125 µg)/test in 100 µL final volume. Cell number may vary (10⁵–10⁸ cells/test).
Notes:
- IL-21 staining shows donor variability; stimulation times should be optimized.
- Enhanced staining observed in Th17-polarized cells.
- eFluor® 660 replaces Alexa Fluor® 647 (Emission 659 nm, Excitation 633 nm).
- Ensure instrument compatibility with red laser detection.
- Filtration: 0.2 µm post-manufacturing.
Excitation: 633–647 nm
Emission: 668 nm
Laser: Red Laser
Target Information
IL-21 is a 17 kDa immunomodulatory cytokine mainly produced by NKT, Th17, and TFH cells. In TFH cells, IL-21 signals through IL-21R and STAT3, activating Bcl6. It is critical for TFH development and function, promoting B-cell differentiation into plasma and memory cells, stimulating IgG production, and inducing apoptosis in naive B cells.
In Th17 cells, IL-21 upregulates IL-23R through STAT3, IRF4, and RORγt, facilitating maturation via IL-23. It also downregulates Foxp3. IL-21 deficiency protects mice from chemically induced colitis, while elevated IL-21 levels are observed in colitis models.
For Research Use Only.
Not for use in diagnostic procedures or resale without authorization.
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