Thermo Fisher Scientific CD196 (CCR6) Monoclonal Antibody (sirx6), eFluor 660, eBioscience

Applications

Tested Dilution

Publications

Flow Cytometry (Flow)

1 µg/test

View 4 publications 4 publications

Miscellaneous PubMed (Misc)

-

View 1 publication 1 publication

Product Specifications

Species Reactivity

Mouse

Published species

Mouse

Host/Isotype

Rat / IgG2a, kappa

Recommended Isotype Control

Rat IgG2a kappa Isotype Control (eBR2a), eFluor™ 660, eBioscience™

Class

Monoclonal

Type

Antibody

Clone

sirx6

Immunogen

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Conjugate

eFluor™ 660 eFluor™ 660 eFluor™ 660

Excitation/Emission Max

651/668 nm View spectra

Form

Liquid

Concentration

0.2 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2

Contains

0.09% sodium azide

Storage conditions

4° C, store in dark, DO NOT FREEZE!

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_11219682

Product Specific Information

Description: The sirx6 monoclonal antibody reacts with mouse CD196 (CCR6). CD196 is a seven transmembrane G protein-coupled receptor expressed on T, B, dendritic, NK and Langerhans cells. This CC chemokine receptor uniquely binds MIP3a/CCL20, a chemoattractant for dendritic cells, effector/memory T cells, and B cells. CD196 is also involved in host defense and inflammation at epithelial sites. Furthermore, this receptor has been implicated in Th17 differentiation and CD4+FoxP3+ regulatory T cell development.

Due to expression characteristics of some chemokine receptors (shedding, surface cleavage or intracellular cycling), we recommend a fixation step after surface staining. Refer to Applications Tested for more information.

Applications Reported: This sirx6 antibody has been reported for use in flow cytometric analysis.

Applications Tested: This sirx6 antibody has been tested by flow cytometric analysis of mouse splenocytes. Cells are surface stained, then washed with Flow Cytometry Staining Buffer, immediately fixed with IC Fixation Buffer for 20-30 minutes, and then washed with Flow Cytometry Staining Buffer prior to analysis. This can be used at less than or equal to 1 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

eFluor® 660 is a replacement for Alexa Fluor® 647. eFluor® 660 emits at 659 nm and is excited with the red laser (633 nm). Please make sure that your instrument is capable of detecting this fluorochome.

Excitation: 633-647 nm; Emission: 668 nm; Laser: Red Laser.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

CCR6 is a member of the beta chemokine receptor family, which is predicted to be a seven transmembrane protein similar to G protein-coupled receptors. The gene is preferentially expressed by immature dendritic cells and memory T cells. The ligand of this receptor is macrophage inflammatory protein 3 alpha (MIP-3 alpha). This receptor has been shown to be important for B-lineage maturation and antigen-driven B-cell differentiation, and it may regulate the migration and recruitment of dentritic and T cells during inflammatory and immunological responses. Alternatively spliced transcript variants that encode the same protein have been described for this gene.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.