Thermo Fisher Scientific CD40 Monoclonal Antibody (5C3), eBioscience

Thermo Fisher Scientific CD40 Monoclonal Antibody (5C3), eBioscience

Applications and Tested Dilutions

Product Specifications

Additional Available Formats

• Alexa Fluor 700
• APC
• APC-eFluor 780
• eFluor 450
• FITC
• Functional Grade
• NovaFluor Blue 660-120S
• NovaFluor Blue 690
• NovaFluor Red 685
• NovaFluor Red 755
• NovaFluor Yellow 610
• NovaFluor Yellow 660
• NovaFluor Yellow 690
• NovaFluor Yellow 755
• PE
• PE-Cyanine7
• PerCP-eFluor 710
• Super Bright 436
• Super Bright 600
• Super Bright 645
• Super Bright 702
• Request custom conjugation available

Product Specific Information

Description:
The 5C3 monoclonal antibody reacts with human CD40, a 45–50 kDa type I transmembrane glycoprotein. CD40 is a member of the TNFR family and is expressed by B lymphocytes, follicular dendritic cells, thymic epithelium, and a subset of peripheral T cells. It regulates B cell development and maturation by inducing Ig isotype-switching and, in combination with IL-4, protects B cells from apoptosis and promotes proliferation. Interaction of CD40 with CD154 (gp39) on T cells is critical for T-B cell crosstalk and immune regulation.
5C3 is reported to activate B cells in in vitro functional assays.

Applications Reported:

• Flow cytometric analysis
• Immunohistochemical staining of frozen tissue sections
• In vitro functional studies (use Functional Grade purified 5C3, Product #16-0409)

Applications Tested:
Tested by flow cytometric analysis of normal human peripheral blood cells. Recommended ≤1 µg per test (100 µL final volume). Cell number may vary from 10⁵–10⁸ cells/test. Antibody titration is recommended for optimal performance.

Purity: >90% (SDS-PAGE)
Aggregation: <10% (HPLC)
Filtration: 0.2 µm post-manufacturing filtered

Target Information

CD40 antigen is a single-chain glycoprotein of the TNF/NGF superfamily, homologous to CD30. Expressed on all B cells except plasma cells, and also on epithelial cells, carcinomas, and lymphoid dendritic cells.
CD40 is essential for immune and inflammatory responses including T cell-dependent immunoglobulin class switching, memory B cell development, and germinal center formation.
AKNA transcription factor regulates CD40 expression; TNFR2 acts as a signal transduction mediator.
CD40-CD154 interaction is implicated in amyloid-beta-induced microglial activation, an early event in Alzheimer’s disease pathogenesis.
Associated diseases: Type 3 Hyper-IgM immunodeficiency, CD40 ligand deficiency.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.