Thermo Fisher Scientific CD40 Monoclonal Antibody (5C3), eBioscience
인간 CD40 항원을 인식하는 단클론 항체로, Flow Cytometry 및 IHC(Frozen) 분석에 적합합니다. B 세포 활성화 및 면역 조절 연구에 활용되며, 0.5 mg/mL 농도의 액상 형태로 제공됩니다. 4°C에서 보관하며 다양한 형광 및 기능성 등급 포맷으로도 제공됩니다.
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Thermo Fisher Scientific CD40 Monoclonal Antibody (5C3), eBioscience
Applications and Tested Dilutions
| Application | Tested Dilution | Publications |
|---|---|---|
| Immunohistochemistry (Frozen) (IHC (F)) | Assay-dependent | - |
| Flow Cytometry (Flow) | 1 µg/test | 11 publications |
| In vitro Assay (IV) | - | 1 publication |
| Miscellaneous PubMed (Misc) | - | 1 publication |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Published Species | Human |
| Host / Isotype | Mouse / IgG1, kappa |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 5C3 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.5 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2 |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_467231 |
Additional Available Formats
- Alexa Fluor 700
- APC
- APC-eFluor 780
- eFluor 450
- FITC
- Functional Grade
- NovaFluor Blue 660-120S
- NovaFluor Blue 690
- NovaFluor Red 685
- NovaFluor Red 755
- NovaFluor Yellow 610
- NovaFluor Yellow 660
- NovaFluor Yellow 690
- NovaFluor Yellow 755
- PE
- PE-Cyanine7
- PerCP-eFluor 710
- Super Bright 436
- Super Bright 600
- Super Bright 645
- Super Bright 702
- Request custom conjugation available
Product Specific Information
Description:
The 5C3 monoclonal antibody reacts with human CD40, a 45–50 kDa type I transmembrane glycoprotein. CD40 is a member of the TNFR family and is expressed by B lymphocytes, follicular dendritic cells, thymic epithelium, and a subset of peripheral T cells. It regulates B cell development and maturation by inducing Ig isotype-switching and, in combination with IL-4, protects B cells from apoptosis and promotes proliferation. Interaction of CD40 with CD154 (gp39) on T cells is critical for T-B cell crosstalk and immune regulation.
5C3 is reported to activate B cells in in vitro functional assays.
Applications Reported:
- Flow cytometric analysis
- Immunohistochemical staining of frozen tissue sections
- In vitro functional studies (use Functional Grade purified 5C3, Product #16-0409)
Applications Tested:
Tested by flow cytometric analysis of normal human peripheral blood cells. Recommended ≤1 µg per test (100 µL final volume). Cell number may vary from 10⁵–10⁸ cells/test. Antibody titration is recommended for optimal performance.
Purity: >90% (SDS-PAGE)
Aggregation: <10% (HPLC)
Filtration: 0.2 µm post-manufacturing filtered
Target Information
CD40 antigen is a single-chain glycoprotein of the TNF/NGF superfamily, homologous to CD30. Expressed on all B cells except plasma cells, and also on epithelial cells, carcinomas, and lymphoid dendritic cells.
CD40 is essential for immune and inflammatory responses including T cell-dependent immunoglobulin class switching, memory B cell development, and germinal center formation.
AKNA transcription factor regulates CD40 expression; TNFR2 acts as a signal transduction mediator.
CD40-CD154 interaction is implicated in amyloid-beta-induced microglial activation, an early event in Alzheimer’s disease pathogenesis.
Associated diseases: Type 3 Hyper-IgM immunodeficiency, CD40 ligand deficiency.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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