Thermo Fisher Scientific CD40 Monoclonal Antibody (1C10), eBioscience
Mouse CD40 단백질을 인식하는 Rat IgG2a 형 단클론 항체로, Flow Cytometry, IHC, ICC, IP 등에 사용 가능. B 세포 발달 및 면역 조절 연구에 적합하며, in vitro/in vivo에서 작용 활성 보고됨. 4°C 보관, 0.5 mg/mL 농도의 액상 제형.
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Thermo Fisher Scientific CD40 Monoclonal Antibody (1C10), eBioscience
Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Immunohistochemistry (IHC) | - | 1 publication |
| Immunohistochemistry (Frozen) (IHC (F)) | Assay-Dependent | - |
| Immunocytochemistry (ICC/IF) | - | 1 publication |
| Flow Cytometry (Flow) | 1 µg/test | 49 publications |
| Immunoprecipitation (IP) | Assay-Dependent | - |
| Functional Assay | - | 10 publications |
| In vitro Assay (IV) | - | 2 publications |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Mouse |
| Published Species | Mouse |
| Host / Isotype | Rat / IgG2a, kappa |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 1C10 |
| Conjugate | Unconjugated |
| Available Formats | APC, Biotin, Brilliant UV 661, Brilliant Violet 605, Functional Grade, PE, PE-Cyanine5, PerCP-eFluor 710, Super Bright 436, Super Bright 600, Super Bright 780 |
| Form | Liquid |
| Concentration | 0.5 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2 |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_467223 |
Product Specific Information
The 1C10 monoclonal antibody reacts with mouse CD40, a 45–50 kDa type I transmembrane glycoprotein. CD40 belongs to the TNFR family and is expressed by mouse B lymphocytes, follicular dendritic cells, thymic epithelium, and a subset of peripheral T cells.
CD40 regulates B cell development and maturation by inducing Ig isotype switching. Together with IL-4, it protects B cells from surface Ig-induced apoptosis and promotes proliferation. Interaction of CD40 with its ligand CD154 (gp39) on T cells is important for T-B cell crosstalk and plays a role in costimulation and immune regulation.
The monoclonal antibody 1C10 is reported to have agonistic activity both in vitro and in vivo.
Applications Reported: Used in flow cytometric analysis, immunoprecipitation, and immunohistochemical staining of frozen tissue sections. For functional assays, use Functional Grade purified 1C10 (Product #16-0401-82).
Applications Tested: Tested by flow cytometric analysis of mouse splenocytes. Recommended ≤1 µg per test (100 µL final volume). Cell number may vary (10⁵–10⁸ cells/test). Antibody should be titrated for optimal performance.
Purity: >90% (SDS-PAGE)
Aggregation: <10% (HPLC)
Filtration: 0.2 µm post-manufacturing filtered
Target Information
CD40 is a single-chain glycoprotein of the TNF/NGF receptor superfamily, homologous to CD30. It is expressed on all B cells except plasma cells, and also on certain epithelial cells, carcinomas, and lymphoid dendritic cells.
CD40 is essential for immune and inflammatory responses, including T cell-dependent immunoglobulin class switching, memory B cell development, and germinal center formation. Transcription factor AKNA regulates CD40 expression, facilitating cell interactions. TNFR2 acts as an adaptor in CD40 signaling.
CD40-CD154 interaction is implicated in amyloid-beta-induced microglial activation, an early event in Alzheimer’s disease pathogenesis. Two alternatively spliced transcript variants of CD40 encoding distinct isoforms have been reported.
Associated Diseases: Type 3 Hyper-IgM immunodeficiency, CD40 ligand deficiency.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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