Thermo Fisher Scientific CD41a Monoclonal Antibody (HIP8), eBioscience

Thermo Fisher Scientific CD41a Monoclonal Antibody (HIP8), eBioscience

Applications and Tested Dilutions

Product Specifications

Available Conjugated Formats

• APC
• APC-eFluor 780
• eFluor 450
• FITC
• NovaFluor Blue 510
• NovaFluor Blue 585
• NovaFluor Blue 660-120S
• NovaFluor Yellow 590
• NovaFluor Yellow 660
• PE
• PE-Cyanine7
• PE-eFluor 610
• PerCP-eFluor 710
• Super Bright 436
• Request custom conjugation available

Product Specific Information

The HIP8 monoclonal antibody reacts with the human CD41 molecule (integrin alphaIIb, platelet GPIIb). CD41 non-covalently associates with integrin beta3 (CD61) and is expressed by megakaryocytes and platelets. The CD41/CD61 complex functions as a receptor for fibronectin, fibrinogen, von Willebrand factor, vitronectin, and thrombospondin, mediating platelet aggregation. HIP8 blocks platelet aggregation.

Applications Reported: Flow cytometric analysis and immunohistochemical staining.
Applications Tested: Flow cytometric analysis of human platelets at ≤0.5 µg/test (100 µL final volume, 10⁵–10⁸ cells/test). Antibody titration recommended for optimal assay performance.
Purity: >90% (SDS-PAGE).
Aggregation: <10% (HPLC).
Filtration: 0.2 µm post-manufacturing filtered.

Target Information

CD41 (platelet glycoprotein IIb, ITGA2B) is composed of two subunits—120 kDa alpha and 23 kDa beta—that interact with CD61 in the presence of calcium to form a functional adhesive receptor. It mediates platelet aggregation and binds von Willebrand factor, fibrinogen, fibronectin, and vitronectin upon vascular injury. CD41 is expressed on megakaryocyte-platelet lineage and during early hematopoietic differentiation.
Diseases associated with CD41 dysfunction include Glanzmann Thrombasthenia and Platelet type-16 bleeding disorder.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without authorization.