Thermo Fisher Scientific mGluR2/mGluR3 Polyclonal Antibody
Rabbit polyclonal antibody for detection of mGluR2/mGluR3 proteins. Suitable for WB, IHC (P/F), and IP applications. Recognizes synthetic peptide from C-terminus of human GluR2/3. Supplied as liquid, store at 4°C short term or -20°C long term.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | Assay-dependent |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:50 |
| Immunohistochemistry (Frozen) (IHC (F)) | Assay-dependent |
| Immunoprecipitation (IP) | Assay-dependent |
Product Specifications
| Specification | Description |
|---|---|
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Synthetic peptide from C-terminus of human GluR2/3 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_2549896 |
Product Specific Information
Heat-mediated antigen retrieval is recommended prior to staining, using a 10 mM citrate buffer (pH 6.0) for 10 minutes followed by cooling at room temperature for 20 minutes.
After antigen retrieval, incubate samples with primary antibody for 10 minutes at room temperature.
A suggested positive control is cerebellum tissue.
Target Information
The mGluR proteins (metabotropic Glutamate Receptors) belong to the G-protein-coupled receptor family and are functionally and pharmacologically distinct from the GluR proteins (ionotropic Glutamate Receptors).
Eight known mGluR proteins are mediated by two G-proteins with opposing regulation of adenylate cyclase pathways.
mGluR1 and mGluR5 activate a phosphatidylinositol-calcium second messenger system and generate a calcium-activated chloride current.
Other mGluR subtypes inhibit adenylate cyclase activity.
mGluR-3, which may interact with GRASP, acts as a receptor for glutamate.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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배송/결제/교환/반품 안내
배송 정보
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