Thermo Fisher Scientific HSPA9 Polyclonal Antibody
Rabbit 폴리클로날 HSPA9 항체로 Western blot, IHC, ICC/IF에 사용 가능. 사람 GRP75 단백질의 합성 펩타이드(aa 661-679)를 면역원으로 제작. 다양한 종에 반응 예측됨. 액상 형태, 0.1 mg/mL 농도, 4°C 단기 및 -20°C 장기 보관 권장.
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- PA532436
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Applications
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:1,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:100 |
| Immunocytochemistry (ICC/IF) | 1:200 |
Product Specifications
| Specification | Description |
|---|---|
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Synthetic peptide corresponding to aa 661–679 of human GRP75 protein |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.1 mg/mL |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_2549905 |
Product Specific Information
This antibody is predicted to react with bovine, mouse, porcine, rabbit, and rat based on sequence homology.
Heat-mediated antigen retrieval is recommended prior to staining, using a 10 mM citrate buffer (pH 6.0) for 10 minutes followed by cooling at room temperature for 20 minutes.
Following antigen retrieval, incubate samples with primary antibody for 10 minutes at room temperature.
A suggested positive control is colon carcinoma.
Target Information
The HSP70 family is composed of four highly conserved proteins: HSP70, HSC70, GRP75, and GRP78.
These proteins serve a variety of roles. GRP75 expression is restricted to the mitochondrial matrix and aids in the translocation and folding of nascent polypeptide chains of both nuclear and mitochondrial origin.
GRP75 and GRP78 are unresponsive to heat stress and are induced by glucose deprivation.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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(PA5-32436_HSPA9_P38646-1_Rabbit.svg, PA5-32436_HSPA9_P38646-1_Rabbit_PDP.jpeg)
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