Thermo Fisher Scientific DIABLO Monoclonal Antibody (78-1-118)

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500

-

Immunocytochemistry (ICC/IF)

5 µg/mL

-

Immunoprecipitation (IP)

1:10-1:500

-

Product Specifications

Species Reactivity

Human, Mouse, Rat

Host/Isotype

Mouse / IgG1/IgG3

Class

Monoclonal

Type

Antibody

Clone

78-1-118

Immunogen

Recombinant mature human Smac/DIABLO (lacks amino acids 1-55 present in Smac/DIABLO precursor) if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:SMAC,uniProtId:Q9NR28-1,ncbiNodeId:9606,antigenRange:1-55,antigenLength:239,antigenImageFileName:MA1-16846_SMAC_Q9NR28-1_House_mouse.svg,antigenImageFileNamePDP:MA1-16846_SMAC_Q9NR28-1_House_mouse_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

Conc. Not Determined

Storage buffer

ascites

Contains

0.1% sodium azide

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Wet ice

RRID

AB_568516

Product Specific Information

Suggested positive control: 293 whole cell lysate.

Target Information

DIABLO (smac), a mitochondrial protein, activates various forms of apoptosis. This activation may be due to the neutralization of one or more members of the IAP family of apoptosis inhibitory proteins. Smac exits the mitochondria and enter the cytosol during certain apoptosis triggered events. Mitochondrial-mediated apoptosis is important in animal development and tissue homeostasis, with alterations resulting in a range of malignant disorders. Upon apoptotic stimuli, the mitochondrial proteins cytochrome c and Smac/DIABLO are released into the cytosol. The release of these proteins, however, occurs via different mechanisms. Smac/DIABLO has also been found to play a key role in regulating the sensitization of cancer cells to apoptosis (both immune and drug-induced).

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.