Thermo Fisher Scientific Blimp-1 Monoclonal Antibody (3H2-E8)

Applications

Tested Dilution

Publications

Western Blot (WB)

1:1,000

-

Immunohistochemistry (IHC)

-

View 1 publication 1 publication

Immunohistochemistry (Paraffin) (IHC (P))

1:200

-

Immunohistochemistry (Frozen) (IHC (F))

Assay-Dependent

-

Immunocytochemistry (ICC/IF)

1:50-1:200

-

Flow Cytometry (Flow)

8 µg/mL

-

ELISA (ELISA)

1:100-1:2,000

-

Immunoprecipitation (IP)

Assay-Dependent

-

ChIP assay (ChIP)

1 µg/10 µg of protein

-

Miscellaneous PubMed (Misc)

-

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human, Mouse, Pig

Published species

Mouse, Pig

Host/Isotype

Mouse / IgG1

Class

Monoclonal

Type

Antibody

Clone

3H2-E8

Immunogen

A fragment of the mouse Blimp-1 (residues 199-409). if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:Blimp-1,uniProtId:Q60636-1,ncbiNodeId:10090,antigenRange:199-409,antigenLength:856,antigenImageFileName:MA1-16874_Blimp-1_Q60636-1_House_mouse.svg,antigenImageFileNamePDP:MA1-16874_Blimp-1_Q60636-1_House_mouse_PDP.jpeg,sortOrder:2}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

View additional formats

• DyLight 488
• DyLight 550
• DyLight 650
• HRP
• Request custom conjugation

Form

Liquid

Concentration

1 mg/mL

Purification

Protein G

Storage buffer

PBS

Contains

0.02% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2237566

Product Specific Information

In Western Blot, this antibody recognizes a band at ~98 kDa and may recognize one at ~80 kDa (the beta form).

Antigen retrieval is recommended (EDTA buffer, microwave) prior to IHC on paraffin tissues.

This antibody does not immunoprecipitate.For IHC and ICC/IF a dilution of 1:200 is recommended with tyramide amplification; this antibody demonstrates nuclear staining.

Suggested positive control: antigen standard for PRDM1 (transient overexpression lysate), P3X (murine plasmocytoma) cell lysates.

Target Information

Blimp-1 (B lymphocyte maturation protein-1) is a nuclear protein with five zinc fingers in its carboxy terminus. It is a transcriptional repressor that is both required and sufficient to trigger terminal differentiation of B lymphocytes and monocyte/macrophages. Blimp-1 is expressed in, and may be required for terminally differentiated epithelial cells, retinal neurons and other lineage cells. It has also been implicated in the repression of beta-interferon (IFN-beta) and c-myc gene expression.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.