Thermo Fisher Scientific Blimp-1 Monoclonal Antibody (3H2-E8)
Blimp-1 단백질을 인식하는 Mouse IgG1 단클론 항체로, WB, IHC, ICC/IF, Flow Cytometry 등 다양한 응용에 사용 가능. Human, Mouse, Pig 반응성. Protein G 정제, 1 mg/mL 농도, PBS 버퍼에 0.02% sodium azide 포함. 연구용 전용 제품.
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Thermo Fisher Scientific Blimp-1 Monoclonal Antibody (3H2-E8)
Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 1:1,000 | - |
| Immunohistochemistry (IHC) | - | 1 publication |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:200 | - |
| Immunohistochemistry (Frozen) (IHC (F)) | Assay-Dependent | - |
| Immunocytochemistry (ICC/IF) | 1:50–1:200 | - |
| Flow Cytometry (Flow) | 8 µg/mL | - |
| ELISA | 1:100–1:2,000 | - |
| Immunoprecipitation (IP) | Assay-Dependent | - |
| ChIP assay (ChIP) | 1 µg/10 µg of protein | - |
| Miscellaneous PubMed (Misc) | - | 1 publication |
Product Specifications
| Property | Description |
|---|---|
| Species Reactivity | Human, Mouse, Pig |
| Published Species | Mouse, Pig |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 3H2-E8 |
| Immunogen | A fragment of the mouse Blimp-1 (residues 199–409) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein G |
| Storage Buffer | PBS |
| Contains | 0.02% sodium azide |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2237566 |
Available Conjugated Formats
Product Specific Information
- In Western Blot, recognizes a band at ~98 kDa and may also detect one at ~80 kDa (beta form).
- Antigen retrieval (EDTA buffer, microwave) is recommended prior to IHC on paraffin tissues.
- Does not immunoprecipitate.
- For IHC and ICC/IF, a 1:200 dilution with tyramide amplification is recommended; demonstrates nuclear staining.
- Suggested positive controls: antigen standard for PRDM1 (transient overexpression lysate), P3X (murine plasmocytoma) cell lysates.
Target Information
Blimp-1 (B lymphocyte maturation protein-1) is a nuclear transcriptional repressor containing five zinc fingers in its carboxy terminus. It is essential for terminal differentiation of B lymphocytes and monocyte/macrophages. Blimp-1 is expressed in terminally differentiated epithelial cells, retinal neurons, and other lineage cells, and is involved in repression of IFN-beta and c-myc gene expression.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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