Thermo Fisher Scientific IRF3 Polyclonal Antibody

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500 - 1:2,000

-

Immunocytochemistry (ICC/IF)

1:50 - 1:200

-

Product Specifications

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 100-200 of human IRF3 if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:IRF3,uniProtId:Q14653-1,ncbiNodeId:9606,antigenRange:100-200,antigenLength:427,antigenImageFileName:PA5-120117_IRF3_Q14653-1_Rabbit.svg,antigenImageFileNamePDP:PA5-120117_IRF3_Q14653-1_Rabbit_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

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Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.16 mg/mL

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Wet ice

RRID

AB_2913689

Product Specific Information

Positive test controls include: THP-1, HT-29. The target is usually found in the following locations: Cytoplasm, Nucleus.

Immunogen sequence: PHDPHKIYEF VNSGVGDFSQ PDTSPDTNGG GSTSDTQEDI LDELLGNMVL APLPDPGPPS LAVAPEPCPQ PLRSPSLDNP TPFPNLGPSE NPLKRLLVPG E

Target Information

IFR3 (interferon regulatory factor 3) is a key transcriptional regulator of type I interferon (IFN)-dependent immune responses. It plays a critical role in the innate immune response against DNA and RNA viruses. IFR3 regulates the transcription of type I IFN genes (IFN-alpha and IFN-beta) and IFN-stimulated genes (ISG) by binding to an interferon-stimulated response element (ISRE) in their promoters. It acts as a more potent activator of the IFN-beta gene than the IFN-alpha gene and plays a critical role in both the early and late phases of the IFNA/B gene induction. IFR3 is found in an inactive form in the cytoplasm of uninfected cells and following viral infection, double-stranded RNA, or toll-like receptor signaling. It is phosphorylated by IKBKE and TBK1 kinases. This induces a conformational change, leading to its dimerization and nuclear localization and association with CREB binding protein to form dsRNA-activated factor 1 (DRAF1), a complex which activates the transcription of the type I IFN and ISG genes. Mutations in the gene can result in encephalopathy, acute, infection-induced, herpes-specific 7.

⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.