Thermo Fisher Scientific MCM7 Polyclonal Antibody
MCM7 단백질을 인식하는 Rabbit Polyclonal 항체로, Western blot, ICC/IF, ELISA, IP 등 다양한 응용에 적합합니다. 인간 및 생쥐 시료에 반응하며, 핵 내 단백질 검출에 유용합니다. 고순도 친화 크로마토그래피 정제 및 안정한 글리세롤 보존 용액 형태로 제공됩니다.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:1,000 |
| Immunocytochemistry (ICC/IF) | 1:50–1:200 |
| ELISA | 1 µg/mL |
| Immunoprecipitation (IP) | 0.5–4 µg antibody for 200–400 µg extracts |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Recombinant fusion protein containing a sequence corresponding to amino acids 1–140 of human MCM7 (NP_005907.3) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.85 mg/mL |
| Purification | Affinity Chromatography |
| Storage Buffer | PBS, pH 7.3, with 50% glycerol |
| Contains | 0.05% ProClin 300 |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Wet ice |
| RRID | AB_2913690 |
Product Specific Information
- Positive test controls: HeLa, HepG2, NIH/3T3, U-87MG
- Subcellular localization: Nucleus
Immunogen sequence:
MALKDYALEK EKVKKFLQEF YQDDELGKKQ FKYGNQLVRL AHREQVALYV DLDDVAEDDP ELVDSICENA RRYAKLFADA VQELLPQYKE REVVNKDVLD VYIEHRLMME QRSRDPGMVR SPQNQYPAEL MRRFELYFQG
Target Information
MCM7 encodes a protein that is one of the highly conserved mini-chromosome maintenance proteins (MCM) essential for the initiation of eukaryotic genome replication. The hexameric MCM complex is a key component of the pre-replication complex (pre_RC) and participates in replication fork formation and recruitment of other DNA replication-related proteins. The MCM complex, including MCM2, 4, 6, and 7, possesses DNA helicase activity and functions as a DNA unwinding enzyme. Cyclin D1-dependent kinase CDK4 associates with this protein and may regulate its interaction with the tumor suppressor RB1/RB. Alternatively spliced transcript variants encoding distinct isoforms have been reported.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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