Thermo Fisher Scientific Histone H2A.X Polyclonal Antibody

Applications and Tested Dilutions

Product Specifications

Product Specific Information

Positive test controls include HeLa, MCF-7, NIH/3T3, Raji, Mouse testis, Mouse thymus, and Rat thymus.
The target is typically localized in the chromosome and nucleus.

Target Information

Histone H2A.X (H2AX) is part of the histone H2A family, one of the four core histones forming the nucleosome core particle.
DNA double-strand breaks (DSBs) induce phosphorylation of serine 139 at the C-terminus of H2AX, producing γ-H2AX, a key DNA damage marker detectable by Western blotting or immunofluorescence.

Phosphorylation is mediated by phosphatidylinositol 3-kinases, potentially including ATM, DNA-PK, and ATR.
H2A.X consists of 143 amino acids and is synthesized in both G1 and S phases. Its mRNA exists in two forms:

• ~1600 bases with polyA tail (basal type)
• ~575 bases without polyA tail (replication type)

Histone H2A.X maps to the 11q23.2–q23.3 region of the human chromosome and contributes to chromatin structure and DNA organization.
It also regulates MDC1, a mediator of DNA damage checkpoint protein, at DNA damage sites.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.