Thermo Fisher Scientific Vimentin Polyclonal Antibody
Vimentin 단백질을 인식하는 Rabbit Polyclonal 항체로, Human, Mouse, Rat 시료에 반응합니다. Western blot, IHC, ELISA 등에 사용 가능하며, 고순도의 Affinity Chromatography 정제 제품입니다. 세포질 내 Vimentin 검출에 적합합니다.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:1,000 |
| Immunohistochemistry (Paraffin) (IHC-P) | 1:50–1:200 |
| ELISA | 1 µg/mL |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Synthetic peptide corresponding to amino acids 300–400 of human Vimentin (NP_003371.2) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 2.05 mg/mL |
| Purification | Affinity Chromatography |
| Storage Buffer | PBS, pH 7.3, with 50% glycerol |
| Contains | 0.02% sodium azide |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Wet ice |
| RRID | AB_2913691 |
Product Specific Information
- Positive test controls: HeLa cells
- Subcellular localization: Cytoplasm
Immunogen sequence:
EAANRNNDAL RQAKQESTEY RRQVQSLTCE VDALKGTNES LERQMREMEE NFAVEAANYQ DTIGRLQDEI QNMKEEMARH LREYQDLLNV KMALDIEIAT Y
Target Information
Vimentin is a developmentally regulated intermediate filament protein (IFP) found in cells of mesenchymal origin. It is involved in intracellular transport of proteins between the nucleus and plasma membrane. Vimentin, along with desmin, is expressed during early cellular development and later replaced by tissue-specific IFPs. It plays a role in steroid synthesis by regulating lipid droplet interactions and cholesterol transport within mitochondria. Phosphorylation of vimentin by protein kinase leads to cytoskeletal remodeling that supports these processes.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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