Thermo Fisher Scientific CD3 Monoclonal Antibody (UCHT1), eFluor 450, eBioscience
사람 CD3ε를 인식하는 UCHT1 단클론 항체로, T세포 분석용 유세포분석(Flow Cytometry)에 적합합니다. eFluor 450 형광체로 결합되어 보라색 레이저(405 nm)로 여기됩니다. 액상 형태로 제공되며, 4°C 암소 보관이 필요합니다.
- 카탈로그번호
- 48-0038-4x (2개 옵션)
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- pk
카탈로그
2개 옵션 · 카탈로그 번호를 클릭하면 복사됩니다Thermo Fisher Scientific · Thermo Fisher Scientific CD3 Monoclonal Antibody (UCHT1), eFluor 450, eBioscience
Applications
- Flow Cytometry (Flow): 5 µL (0.5 µg)/test
- View 30 publications
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Published Species | Human, Mouse |
| Host / Isotype | Mouse / IgG1, kappa |
| Recommended Isotype Control | Mouse IgG1 kappa Isotype Control (P3.6.2.8.1), eFluor™ 450, eBioscience™ |
| Class | Monoclonal |
| Type | Antibody |
| Clone | UCHT1 |
| Conjugate | eFluor™ 450 |
| Excitation / Emission Max | 405 / 445 nm |
| Form | Liquid |
| Concentration | 5 µL/Test |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2, with 0.2% BSA |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C, store in dark, DO NOT FREEZE |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_1518800 |
Available Formats
- Unconjugated
- Alexa Fluor 532, 561, 660, 700
- APC, APC-eFluor 780
- Biotin
- Brilliant UV & Violet series
- FITC
- PE, PE-Cyanine5, PE-Cyanine7, PE-eFluor 610
- Super Bright series
- NovaFluor Blue/Red/Violet/Yellow series
- Functional Grade
- Request custom conjugation
Product Specific Information
The UCHT1 monoclonal antibody reacts with human CD3ε, a 20 kDa subunit of the TCR complex. Together with CD3γ and CD3δ, the epsilon chain is required for proper assembly, trafficking, and surface expression of the TCR complex. CD3 is expressed by thymocytes and all mature T cells.
Crosslinking of TCR via immobilized UCHT1 initiates intracellular signaling leading to cellular activation and proliferation.
- Applications Reported: Flow cytometric analysis
- Applications Tested: Pre-titrated and validated for human peripheral blood cells; use at 5 µL (0.5 µg) per 100 µL test volume (10⁵–10⁸ cells/test)
Fluorophore Information:
eFluor™ 450 is a replacement for Pacific Blue™, excited at 405 nm and emitting at 445 nm. Ensure instrument compatibility with this fluorochrome.
Filtration: 0.2 µm post-manufacturing filtered.
Target Information
The CD3 complex transduces antigen-recognition signals into T cell cytoplasm and regulates TCR surface expression. It includes CD3γ, CD3δ, CD3ε, and CD3ζ subunits, encoded on human chromosome 11. These subunits associate with cytoplasmic signaling molecules via tyrosine-based motifs, mediating T cell activation, survival, and proliferation.
CD3 is expressed on 68–82% of normal peripheral blood lymphocytes and 65–85% of thymocytes, but not on B or NK cells. Reduced T cell percentages may occur in autoimmune diseases. Defects in CD3 genes are linked to CD3 immunodeficiency.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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