Thermo Fisher Scientific CD3 Monoclonal Antibody (UCHT1), eFluor 450, eBioscience
Human CD3e 인식용 UCHT1 단클론 항체로, eFluor 450 형광체가 결합된 제품입니다. Flow cytometry에 최적화되어 있으며, Violet laser(405 nm)로 여기되어 445 nm에서 방출됩니다. T세포 활성화 연구 및 면역세포 분석에 적합합니다.
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Thermo Fisher Scientific CD3 Monoclonal Antibody (UCHT1), eFluor 450, eBioscience
Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Immunohistochemistry (IHC) | - | View 1 publication |
| Flow Cytometry (Flow) | 0.5 µg/test | View 32 publications |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human |
| Published Species | Human, Mouse |
| Host/Isotype | Mouse / IgG1, kappa |
| Recommended Isotype Control | Mouse IgG1 kappa Isotype Control (P3.6.2.8.1), eFluor™ 450 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | UCHT1 |
| Conjugate | eFluor™ 450 |
| Excitation/Emission Max | 405/445 nm |
| Form | Liquid |
| Concentration | 0.2 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2 |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C, store in dark, DO NOT FREEZE |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_1518799 |
Available Formats
- Unconjugated
- Alexa Fluor 532, 561, 660, 700
- APC, APC-eFluor 780
- Biotin, Brilliant UV/Violet series
- FITC, PE, PE-Cyanine5, PE-Cyanine7
- Super Bright series
- NovaFluor Blue/Red/Violet/Yellow series
- Functional Grade
- Request custom conjugation available
Product Specific Information
The UCHT1 monoclonal antibody reacts with human CD3e, a 20 kDa subunit of the TCR complex. The epsilon chain, along with CD3 gamma and delta, is essential for proper assembly and surface expression of the TCR complex. CD3 is expressed by thymocytes and all mature T cells.
Crosslinking of TCR via immobilized UCHT1 initiates intracellular signaling resulting in activation and proliferation.
This antibody is pre-titrated for flow cytometric analysis of normal human peripheral blood cells and can be used at 5 µL (0.5 µg) per test in a final volume of 100 µL.
eFluor™ 450 is a replacement for Pacific Blue™, excited at 405 nm and emits at 445 nm. Ensure instrument compatibility with this fluorochrome.
Filtration: 0.2 µm post-manufacturing filtered.
Target Information
The CD3 complex transduces antigen-recognition signals into T cells and regulates TCR surface expression. It consists of CD3 gamma, delta, epsilon, and zeta subunits, all members of the immunoglobulin superfamily encoded on chromosome 11.
CD3 is present on 68–82% of peripheral blood lymphocytes and 65–85% of thymocytes, but absent on B and NK cells. Abnormal CD3 expression can be associated with autoimmune diseases or immunodeficiency.
For Research Use Only. Not for use in diagnostic procedures or resale without authorization.
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