Thermo Fisher Scientific CD3e Monoclonal Antibody (eBio500A2 (500A2)), eFluor 450, eBioscience

Thermo Fisher Scientific CD3e Monoclonal Antibody (eBio500A2 (500A2)), eFluor 450

Applications and Tested Dilution

• Immunohistochemistry (IHC): Not specified
  View 1 publication
• Flow Cytometry (Flow): 1 µg/test
  View 9 publications

Product Specifications

Additional Formats Available:

• Unconjugated
• Alexa Fluor 700
• APC-eFluor 780
• Biotin
• FITC
• Functional Grade
• PE
• PerCP-eFluor 710
• Super Bright 436
• Custom conjugation available

Product Specific Information

The eBio500A2 monoclonal antibody reacts with the 25 kD epsilon subunit of the mouse CD3 complex. CD3 subunits (γ, δ, ε) are essential for assembly, trafficking, and surface expression of the TCR complex. CD3 is expressed by thymocytes and mature T cells, including NKT cells. Binding of eBio500A2 to CD3 initiates intracellular signaling, leading to cellular activation and proliferation.

This antibody has been tested for flow cytometric analysis of mouse splenocytes and can be used at ≤1 µg/test (100 µL final volume, 10⁵–10⁸ cells/test). Optimal titration is recommended.

Fluorophore Information:

• eFluor™ 450 is an alternative to Pacific Blue™
• Excitation: 405 nm
• Emission: 445 nm
• Laser: Violet
• Filtered post-manufacturing (0.2 µm)

Target Information

The CD3 complex transduces antigen-recognition signals into T cells and regulates TCR surface expression. It consists of CD3γ, CD3δ, CD3ε, and CD3ζ subunits, encoded by closely linked genes on chromosome 11. These subunits contain cytoplasmic tails with tyrosine-based motifs for signal transduction.

CD3 is expressed on:

• 68–82% of normal peripheral blood lymphocytes
• 65–85% of thymocytes
• Purkinje cells in the cerebellum
  Not expressed on B or NK cells.

Defects in CD3 genes are associated with CD3 immunodeficiency and may be linked to autoimmune diseases.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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