Thermo Fisher Scientific CD3 Monoclonal Antibody (OKT3), eFluor 450, eBioscience
인간 CD3 복합체의 epsilon 서브유닛을 인식하는 OKT3 단클론 항체. eFluor 450 형광체로 결합되어 유세포분석에 최적화. T세포 활성화 및 면역반응 연구에 적합. 405 nm 여기, 445 nm 방출 특성.
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Thermo Fisher Scientific CD3 Monoclonal Antibody (OKT3), eFluor 450, eBioscience
Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Immunohistochemistry (IHC) | - | 2 |
| Immunocytochemistry (ICC/IF) | - | 4 |
| Flow Cytometry (Flow) | 5 µL (1 µg)/test | 51 |
| Functional Assay | - | 1 |
| Immunomicroscopy (IM) | - | 1 |
| T-Cell Activation (TCA) | - | 5 |
| Miscellaneous PubMed (Misc) | - | 2 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Published Species | Human, Mouse |
| Host/Isotype | Mouse / IgG2a, kappa |
| Recommended Isotype Control | Mouse IgG2a kappa Isotype Control (eBM2a), eFluor™ 450 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | OKT3 |
| Conjugate | eFluor™ 450 |
| Excitation/Emission Max | 405/445 nm |
| Form | Liquid |
| Concentration | 5 µL/Test |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2, with 0.2% BSA |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C, store in dark, DO NOT FREEZE |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_1272125 |
Additional Formats Available
- Unconjugated
- Alexa Fluor 488
- Alexa Fluor 700
- APC
- APC-eFluor 780
- Biotin
- Brilliant UV and Violet series
- eFluor 506, eFluor 660
- FITC
- Functional Grade
- NovaFluor Blue/Yellow series
- PE, PE-Cyanine7, PE-eFluor 610
- PerCP-Cyanine5.5, PerCP-eFluor 710
- Super Bright series (436–780)
- Custom conjugation available
Product Specific Information
The OKT3 monoclonal antibody reacts with an epitope on the epsilon-subunit within the human CD3 complex. It exhibits potent immunosuppressive properties and is effective in the treatment of renal, heart, and liver allograft rejection. CD3 subunits (gamma, delta, epsilon) are essential for TCR complex assembly and surface expression. CD3 is expressed by thymocytes and all mature T cells.
Crosslinking of TCR initiates intracellular signaling leading to activation and proliferation.
This antibody is validated for flow cytometric analysis of normal human peripheral blood cells at 5 µL (1 µg) per test (100 µL final volume).
Cell number may vary from 10⁵ to 10⁸ cells per test.
Fluorophore Information:
eFluor® 450 is an alternative to Pacific Blue®, excited at 405 nm and emits at 445 nm. Ensure instrument compatibility with violet laser detection.
Filtration: 0.2 µm post-manufacturing filtered.
Target Information
The CD3 complex transduces antigen-recognition signals into T cells and regulates surface expression of the TCR complex. CD3 subunits (gamma, delta, epsilon, zeta) are structurally related members of the immunoglobulin superfamily encoded on chromosome 11. They associate with cytoplasmic signal transduction molecules via tyrosine-based motifs.
CD3 is expressed on 68–82% of peripheral blood lymphocytes and 65–85% of thymocytes, but not on B or NK cells. Decreased T lymphocytes may occur in autoimmune diseases.
Defects in CD3 genes are linked to CD3 immunodeficiency.
For Research Use Only. Not for use in diagnostic procedures or resale without authorization.
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