Thermo Fisher Scientific CD11b Monoclonal Antibody (ICRF44), Super Bright 645, eBioscience
인간 CD11b 인식 단클론 항체(ICRF44)로 Super Bright 645 형광체 결합. 유세포분석(Flow Cytometry)에 최적화되어 있으며, 단핵구·과립구·NK세포 등의 표면 CD11b 검출에 사용. 405 nm 레이저로 여기, 645 nm에서 방출. 연구용으로만 사용.
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Thermo Fisher Scientific CD11b Monoclonal Antibody (ICRF44), Super Bright 645, eBioscience™
Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Immunohistochemistry (IHC) | - | View 1 publication |
| Flow Cytometry (Flow) | 5 µL (0.125 µg)/test | View 20 publications |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Baboon, Chimpanzee, Cynomolgus monkey, Human, Rhesus monkey |
| Published Species | Not Applicable |
| Host / Isotype | Mouse / IgG1, kappa |
| Recommended Isotype Control | Mouse IgG1 kappa Isotype Control (P3.6.2.8.1), Super Bright™ 645, eBioscience™ |
| Class | Monoclonal |
| Type | Antibody |
| Clone | ICRF44 |
| Conjugate | Super Bright™ 645 |
| Excitation/Emission Max | 414 / 645 nm |
| Form | Liquid |
| Concentration | 5 µL/Test |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2, with BSA |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C, store in dark, DO NOT FREEZE |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2717083 |
Available Conjugate Formats
- Unconjugated
- Alexa Fluor 700
- APC
- APC-eFluor 780
- Biotin
- Brilliant UV 395 / 496 / 563 / 615 / 661 / 737 / 805
- Brilliant Violet 421 / 480 / 605 / 650 / 711 / 786
- eFluor 506
- FITC
- NovaFluor Blue / Red / Violet / Yellow series
- PE, PE-Cyanine5, PE-Cyanine7, PE-eFluor 610
- PerCP-eFluor 710
- Super Bright 436 / 600 / 702 / 780
- Custom conjugation available upon request
Product Specific Information
The ICRF44 monoclonal antibody reacts with human CD11b, a 165 kDa adhesion molecule associated with integrin beta2 (CD18). It is expressed on monocytes, granulocytes, activated lymphocytes, and a subset of NK cells. CD11b functions as a receptor for ICAM family members (CD54, CD102, CD50) and iC3b, playing a crucial role in cell-cell and cell-matrix interactions.
Applications Reported: Flow cytometric analysis.
Applications Tested: Pre-diluted and tested by flow cytometry using normal human peripheral blood cells. Recommended use is 5 µL (0.125 µg) per test (100 µL final volume, 10⁵–10⁸ cells/test).
Fluorochrome Information:
Super Bright 645 is a tandem dye excited by the violet laser (405 nm) and emits at 645 nm. Use a 660/20 bandpass filter. Protect from light due to photo-induced oxidation sensitivity.
Staining Recommendations:
When using multiple Super Bright dye-conjugated antibodies, use Super Bright Complete Staining Buffer (Product # SB-4401) to minimize non-specific polymer interactions.
Fixation:
Samples can be stored up to 3 days at 4°C in the dark using IC Fixation Buffer (Product # 00-8222) or 1-step Fix/Lyse Solution (Product # 00-5333).
Excitation/Emission: 405 nm / 645 nm
Laser: Violet Laser
Super Bright Polymer Dyes are sold under license from Becton, Dickinson and Company.
Target Information
CD11b (integrin alpha-M, ITGAM) forms a heterodimer with CD18 (integrin beta-2) known as Mac-1 or CR3. It is expressed on monocytes/macrophages, granulocytes, activated lymphocytes, NK cells, dendritic cells, and microglia. CD11b/CD18 binds ICAM-1, ICAM-2, ICAM-4, CD14, CD50, CD23, heparin, iC3b, fibrinogen, and Factor X, mediating cell adhesion and immune response. Expression varies with cell type and maturation state and is a key microglial marker.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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