Thermo Fisher Scientific CD14 Monoclonal Antibody (61D3), Super Bright 645, eBioscience

Applications and Tested Dilution

Product Specifications

Additional Formats Available

Unconjugated, Alexa Fluor 488, Alexa Fluor 532, Alexa Fluor 660, Alexa Fluor 700, APC, APC-eFluor 780, Biotin, Brilliant UV 395/496/615/661/737/805, Brilliant Violet 421/480/605/650/711/786, eFluor 450/506, FITC, Functional Grade, NovaFluor Blue 510/555/585/610-30S/660-40S/800, NovaFluor Yellow 570/660, PE, PE-Cyanine5, PE-Cyanine7, PE-eFluor 610, PerCP-Cyanine5.5, PerCP-eFluor 710, Super Bright 436/600/702/780
Request custom conjugation

Product Specific Information

The 61D3 monoclonal antibody reacts with human CD14, a 53–55 kDa GPI-linked glycoprotein expressed on monocytes, interfollicular macrophages, and some dendritic cells. Complexes of LPS and LBP bind with high affinity to monocytes through surface CD14.

Applications Reported: Flow cytometric analysis
Applications Tested: Pre-titrated and tested by flow cytometry on normal human peripheral blood cells. Recommended use: 5 µL (0.5 µg) per test in 100 µL final volume.

Fluorochrome Information:
Super Bright 645 is a tandem dye excited with the violet laser (405 nm) and emits at 645 nm. Recommended detection filter: 660/20 bandpass.

Staining Buffer Recommendation:
When using multiple Super Bright dye-conjugated antibodies, use Super Bright Complete Staining Buffer (Product # SB-4401) to minimize non-specific polymer interactions.

Light Sensitivity:
Protect vial and stained samples from light due to photo-induced oxidation sensitivity.

Fixation:
Samples may be stored up to 3 days at 4°C in the dark using IC Fixation Buffer (Product # 00-8222) or 1-step Fix/Lyse Solution (Product # 00-5333) with minimal impact on brightness and compensation.

Excitation: 405 nm
Emission: 645 nm
Laser: Violet

Super Bright Polymer Dyes are sold under license from Becton, Dickinson and Company.

Target Information

CD14 is a 55 kDa GPI-anchored glycoprotein expressed on mature monocytes, macrophages, and neutrophils. It functions as a multifunctional lipopolysaccharide receptor and exists in both membrane-bound and soluble forms. CD14 interacts with lipopolysaccharide-binding protein (LBP) and plays a role in immune response modulation, including recognition of apoptotic cells and mediation of inflammatory processes.
Diseases associated with CD14 dysfunction include Mycobacterium chelonae infection and Croup.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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