Thermo Fisher Scientific CD10 Monoclonal Antibody (eBioCB-CALLA (CB-CALLA)), Super Bright 645, eBioscience

Applications and Tested Dilution

Product Specifications

Available Formats

• Unconjugated
• Alexa Fluor 700
• APC
• APC-eFluor 780
• Biotin
• FITC
• NovaFluor Blue 610-70S
• NovaFluor Blue 660-120S
• NovaFluor Red 700
• NovaFluor Yellow 610
• NovaFluor Yellow 660
• PE
• PE-Cyanine5
• PE-Cyanine7
• PerCP-eFluor 710
• Super Bright 436
• Super Bright 600
• Super Bright 702
• Super Bright 780
• Request custom conjugation

Product Specific Information

The eBioCB-CALLA monoclonal antibody recognizes human CD10 (CALLA, NEP, enkephalinase, Neprilysin), a 100 kDa type II cell surface glycoprotein originally identified on most acute lymphoblastic leukemias (ALL). CD10 is a Zn²⁺-dependent metallo-peptidase involved in the regulation of chemotactic and inflammatory processes involving neutrophils. In B cells, CD10 regulates stromal cell-dependent B lymphopoiesis and is expressed in germinal center B cells. It is also found in enterocytes and renal tubular brush borders.

Applications Reported: Suitable for flow cytometric analysis.
Applications Tested: Pre-diluted for flow cytometry; use 5 µL (0.25 µg) per test (100 µL final volume, 10⁵–10⁸ cells/test).

Super Bright 645 is a tandem dye excited by the violet laser (405 nm) and emits at 645 nm (recommended filter: 660/20 bandpass). Ensure instrument compatibility.

When using multiple Super Bright dye-conjugated antibodies, use Super Bright Complete Staining Buffer (Product # SB-4401) to reduce non-specific interactions.

Light Sensitivity: Protect from light to prevent photo-induced oxidation.
Fixation: Samples can be stored in IC Fixation Buffer (Product # 00-8222) or 1-step Fix/Lyse Solution (Product # 00-5333) for up to 3 days at 4°C in the dark with minimal fluorescence loss.

Excitation: 405 nm
Emission: 645 nm
Laser: Violet Laser

Super Bright Polymer Dyes are sold under license from Becton, Dickinson and Company.

Target Information

CD10 (CALLA) is a cell surface enzyme with neutral metalloendopeptidase activity that inactivates various biologically active peptides. It is expressed on lymphoblastic, Burkitt’s, and germinal center lymphoma cells, immature B cells, and CML cells. CD10 is also found on breast myoepithelial cells, bile canaliculi, fibroblasts, and highly expressed on kidney and gut epithelial brush borders.

CD10 cleaves peptides at hydrophobic residues and inactivates hormones such as glucagon, enkephalins, substance P, neurotensin, oxytocin, and bradykinin. It is a 100 kDa type II transmembrane glycoprotein encoded by a >45 kb gene with alternatively spliced 5′ UTRs.

Diseases associated with CD10 dysfunction include spinocerebellar ataxia 43 and Charcot-Marie-Tooth disease.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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