Thermo Fisher Scientific CEP164 Polyclonal Antibody

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:2,000

-

Immunohistochemistry (Paraffin) (IHC (P))

1:50-1:200

-

Product Specifications

Species Reactivity

Human, Mouse, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Recombinant fusion protein of human CEP164.

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.2, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Wet ice

RRID

AB_2720188

Product Specific Information

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Target Information

CEP164 was initially identified as a centrosomal protein, but other studies have indicated that it also plays a role in the formation of primary cilia, the microtubule-based sensory antennae projecting from the surface of many eukaryotic cells as well as in DNA damage response acting as a mediator protein. CEP164 interacts with both ATR and ATM, proteins that trigger a number of cellular responses including the initiation of DNA damaged-induced cell cycle checkpoints. It is phosphorylated upon replication stress, ultraviolet (UV) radiation, and ionizing radiation silencing of CEP164 significantly reduces the DNA damage-induced phosphorylation of several proteins in the DNA damage-activated signaling cascade and compromises cell survival after UV damage. At least two isoforms of CEP164 are known to exist.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.