Thermo Fisher Scientific CD49f (Integrin alpha 6) Monoclonal Antibody (eBioGoH3 (GoH3)), Biotin, eBioscience

Thermo Fisher Scientific CD49f (Integrin alpha 6) Monoclonal Antibody (eBioGoH3 (GoH3)), Biotin, eBioscience

Applications and Tested Dilutions

Product Specifications

Available Formats

• Unconjugated
• Alexa Fluor 700
• APC
• APC-eFluor 780
• eFluor 450
• FITC
• PE
• PE-Cyanine7
• PerCP-eFluor 710
• Super Bright 436
• Super Bright 600
• Super Bright 780
• Request custom conjugation

Product Specific Information

The eBioGoH3 monoclonal antibody reacts with mouse and human CD49f (Integrin alpha 6), also known as VLA-6 alpha chain and platelet gpIc.
CD49f is a 120 kD transmembrane protein that associates with CD29 (integrin beta 1) to form the VLA-6 complex, and with CD104 (integrin beta 4) to form the alpha6 beta4 complex.

Expression:
Primarily on T cells, monocytes, platelets, epithelial and endothelial cells. Also found on germinal center B cells.

Cross-reactivity:
Human, mouse, and bovine integrin alpha 6.

Functional activity:
Reported to block the binding of integrin alpha 6 to laminin.

Applications Reported:
Flow cytometric analysis and immunohistology staining of frozen tissue sections.

Applications Tested:
Tested by flow cytometric analysis of human peripheral blood cells. Recommended ≤0.25 µg per test (100 µL final volume). Cell number: 10⁵–10⁸ cells/test.
Antibody should be titrated for optimal performance.

Filtration:
0.2 µm post-manufacturing filtered.

Target Information

CD49f (Integrin alpha-6, ITGA6) is the integrin alpha chain alpha 6 protein. Integrins are integral cell-surface proteins composed of alpha and beta chains that combine to form diverse integrin complexes.

• Alpha6 + Beta4 → Integrin TSP180
• Alpha6 + Beta1 → Integrin VLA-6

Functions:

• Mediates cell adhesion and signaling
• Connects extracellular matrix (ECM) to cytoskeletal linker proteins
• Regulates cellular shape, motility, and proliferation
• Plays a structural role in hemidesmosomes

Expression:
Predominantly in epithelial cells.

Integrin Family:

18 alpha and 8 beta subunits identified in mammals, with numerous splice variants.

Research Use:
For Research Use Only. Not for use in diagnostic procedures or resale without authorization.