
Thermo Fisher Scientific CD49f (Integrin alpha 6) Monoclonal Antibody (eBioGoH3 (GoH3)), Biotin, eBioscience
CD49f (Integrin alpha 6) 단클론 항체로 인간 및 마우스 세포에서 교차 반응성을 보입니다. Flow cytometry와 IHC(Frozen)에 적합하며, Biotin으로 표지되어 있습니다. 세포 부착 및 신호전달 연구에 활용 가능하며, 0.5 mg/mL 농도로 액상 형태로 제공됩니다.
- 카탈로그번호
- 13-0495-8x (2개 옵션)
- 판매단위
- pk
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Thermo Fisher Scientific CD49f (Integrin alpha 6) Monoclonal Antibody (eBioGoH3 (GoH3)), Biotin, eBioscience
Applications and Tested Dilutions
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | - | View 1 publication |
| Immunohistochemistry (IHC) | - | View 4 publications |
| Immunohistochemistry (Frozen) (IHC (F)) | Assay-Dependent | - |
| Immunocytochemistry (ICC/IF) | - | View 1 publication |
| Flow Cytometry (Flow) | 0.25 µg/test | View 43 publications |
| Neutralization (Neu) | - | View 2 publications |
Product Specifications
| 항목 | 내용 |
|---|---|
| Published Species | Human, Mouse |
| Host / Isotype | Rat / IgG2a, kappa |
| Recommended Isotype Control | Rat IgG2a kappa Isotype Control (eBR2a), Biotin, eBioscience™ |
| Class | Monoclonal |
| Type | Antibody |
| Clone | eBioGoH3 (GoH3) |
| Conjugate | Biotin |
| Form | Liquid |
| Concentration | 0.5 mg/mL |
| Storage Conditions | 4°C, store in dark, DO NOT FREEZE! |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_1210591 |
Available Formats
- Unconjugated
- Alexa Fluor 700
- APC
- APC-eFluor 780
- eFluor 450
- FITC
- PE
- PE-Cyanine7
- PerCP-eFluor 710
- Super Bright 436
- Super Bright 600
- Super Bright 780
- Request custom conjugation
Product Specific Information
The eBioGoH3 monoclonal antibody reacts with mouse and human CD49f (Integrin alpha 6), also known as VLA-6 alpha chain and platelet gpIc.
CD49f is a 120 kD transmembrane protein that associates with CD29 (integrin beta 1) to form the VLA-6 complex, and with CD104 (integrin beta 4) to form the alpha6 beta4 complex.
Expression:
Primarily on T cells, monocytes, platelets, epithelial and endothelial cells. Also found on germinal center B cells.
Cross-reactivity:
Human, mouse, and bovine integrin alpha 6.
Functional activity:
Reported to block the binding of integrin alpha 6 to laminin.
Applications Reported:
Flow cytometric analysis and immunohistology staining of frozen tissue sections.
Applications Tested:
Tested by flow cytometric analysis of human peripheral blood cells. Recommended ≤0.25 µg per test (100 µL final volume). Cell number: 10⁵–10⁸ cells/test.
Antibody should be titrated for optimal performance.
Filtration:
0.2 µm post-manufacturing filtered.
Target Information
CD49f (Integrin alpha-6, ITGA6) is the integrin alpha chain alpha 6 protein. Integrins are integral cell-surface proteins composed of alpha and beta chains that combine to form diverse integrin complexes.
- Alpha6 + Beta4 → Integrin TSP180
- Alpha6 + Beta1 → Integrin VLA-6
Functions:
- Mediates cell adhesion and signaling
- Connects extracellular matrix (ECM) to cytoskeletal linker proteins
- Regulates cellular shape, motility, and proliferation
- Plays a structural role in hemidesmosomes
Expression:
Predominantly in epithelial cells.
Integrin Family:
18 alpha and 8 beta subunits identified in mammals, with numerous splice variants.
Research Use:
For Research Use Only. Not for use in diagnostic procedures or resale without authorization.
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