Thermo Fisher Scientific CD49e (Integrin alpha 5) Monoclonal Antibody (eBioSAM-1 (SAM-1, SAM1)), Biotin, eBioscience
인간 CD49e (Integrin α5)를 인식하는 단클론 항체로, Biotin으로 표지되어 있습니다. 유세포분석(Flow Cytometry)에 적합하며, 고순도의 Affinity chromatography로 정제되었습니다. PBS 버퍼에 보관하며 4°C, 암소에서 보관합니다.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Flow Cytometry (Flow) | 0.5 µg/test |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Published Species | Human |
| Host / Isotype | Mouse / IgG2b |
| Recommended Isotype Control | Mouse IgG2b kappa Isotype Control (eBMG2b), Biotin, eBioscience™ |
| Class | Monoclonal |
| Type | Antibody |
| Clone | eBioSAM-1 (SAM-1, SAM1) |
| Conjugate | Biotin |
| Form | Liquid |
| Concentration | 0.5 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2 |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C, store in dark, DO NOT FREEZE |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_763503 |
| Filtration | 0.2 µm post-manufacturing filtered |
Available Formats
Product Specific Information
The eBioSAM-1 monoclonal antibody reacts with human integrin alpha 5, also known as fibronectin receptor alpha chain, very late activation antigen 5 alpha, and CD49e. Integrins are composed of an alpha and a beta chain that form functional heterodimers responsible for cell surface-mediated signaling and adhesion.
Integrin alpha 5 undergoes post-translational cleavage in its extracellular domain to yield disulfide-linked light and heavy chains that join with Integrin beta 1 (CD29) to form the fibronectin receptor (VLA-5 complex). It is expressed on thymocytes, T cells, monocytes, platelets, early B cells, and activated B cells.
Applications Reported:
This eBioSAM-1 (SAM-1, SAM1) antibody is reported for use in flow cytometric analysis.
Applications Tested:
Tested by flow cytometric analysis of normal human peripheral blood cells. Recommended use ≤0.5 µg/test (100 µL final volume). Cell number range: 10⁵–10⁸ cells/test. Optimize titration for best performance.
Target Information
The gene product belongs to the integrin alpha chain family. Integrins are heterodimeric membrane proteins composed of alpha and beta chains. The integrin alpha 5 chain undergoes post-translational cleavage to form disulfide-linked light and heavy chains that combine with beta 1 to form the fibronectin receptor. Besides adhesion, integrins also participate in cell-surface mediated signaling.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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