Thermo Fisher Scientific CD51 (Integrin alpha V) Monoclonal Antibody (RMV-7), Biotin, eBioscience

Applications

Tested Dilution

Publications

Immunohistochemistry (IHC)

-

View 2 publications 2 publications

Flow Cytometry (Flow)

1 µg/test

View 14 publications 14 publications

Functional Assay (Functional)

-

View 1 publication 1 publication

Product Specifications

Species Reactivity

Mouse

Published species

Human, Mouse

Host/Isotype

Rat / IgG1, kappa

Recommended Isotype Control

Rat IgG1 kappa Isotype Control (eBRG1), Biotin, eBioscience™

Class

Monoclonal

Type

Antibody

Clone

RMV-7

Conjugate

Biotin Biotin Biotin

View additional formats

• Unconjugated
• PE
• Request custom conjugation

Form

Liquid

Concentration

0.5 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2

Contains

0.09% sodium azide

Storage conditions

4° C, store in dark, DO NOT FREEZE!

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_466477

Product Specific Information

Description: The RMV-7 monoclonal antibody reacts with the mouse CD51 molecule, the integrin alpha v chain. This approximately 120 kDa surface molecule non-covalently associates with the beta subunits of the integrin family including beta3 (CD61), beta1 (CD29), beta5 and beta6 to form receptors for extracellular matrix components. Heterodimers of CD51/CD61 are expressed by platelets, T cells and granulocytes and mediate adhesion to fibrinogen, fibronectin, vitronectin and thrombospondin.

Applications Reported: The RMV-7 antibody has been reported for use in flow cytometric analysis.

Applications Tested: The RMV-7 antibody has been tested by flow cytometric analysis of mouse splenocytes. This can be used at less than or equal to 1 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

ITAGV encodes integrin alpha chain V. Integrins are heterodimeric integral membrane proteins composed of an alpha chain and a beta chain. The I-domain containing integrin alpha V undergoes post-translational cleavage to yield disulfide-linked heavy and light chains, that combine with multiple integrin beta chains to form different integrins. Among the known associating beta chains (beta chains 1,3,5,6, and 8, ITGB1, ITGB3, ITGB5, ITGB6, and ITGB8), each can interact with extracellular matrix ligands, the alpha V beta 3 integrin, perhaps the most studied of these, is referred to as the Vitronectin receptor (VNR). In addition to adhesion, many integrins are known to facilitate signal transduction. The ITGB3 protein product is the integrin beta chain beta 3. Integrins are integral cell-surface proteins composed of an alpha chain and a beta chain. A given chain may combine with multiple partners resulting in different integrins. Integrin beta 3 is found along with the alpha IIb chain in platelets. Integrins are known to participate in cell adhesion as well as cell-surface mediated signalling.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.