Thermo Fisher Scientific EAAC1 Monoclonal Antibody (N180/41), PerCP
EAAC1 단백질을 인식하는 Mouse IgG1 단일클론 항체로 PerCP 형광 표지형입니다. Western blot, ICC/IF, IP 등 다양한 응용에 사용 가능하며, 인간 및 랫트 반응성을 보입니다. 고순도 Protein G 정제, 4°C 보관 조건의 연구용 시약입니다.
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Thermo Fisher Scientific EAAC1 Monoclonal Antibody (N180/41), PerCP
Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:1,000 |
| Immunocytochemistry (ICC/IF) | 1:100 |
| Immunoprecipitation (IP) | Assay-dependent |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Rat |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | N180/41 |
| Immunogen | Fusion protein amino acids 1–524 (full length) of human EAAC1 |
| Conjugate | PerCP |
| Excitation / Emission Max | 482 / 675 nm |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein G |
| Storage Buffer | 95.64 mM phosphate / 2.48 mM MES, pH 7.4, with 0.5 M EDTA |
| Contains | No preservative |
| Storage Conditions | 4°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2932003 |
Additional Formats
Product Specific Information
- 5 µg of MA5-45549 was sufficient for immunoprecipitation of EAAC1 from 50 µg of rat brain lysate.
- Detects approximately 60 kDa.
- This antibody was formerly sold as clone S180-41.
Target Information
L-glutamate is the major excitatory neurotransmitter in mammalian central nervous systems. To maintain glutamate levels below excitotoxic levels, excess glutamate at excitatory synaptic clefts is removed by ion-coupled glutamate transporters. Four glutamate transporters have been identified: GLAST-1, GLT-1, EAAC-1, and EAAT-4.
EAAC-1 is expressed in the CNS and also found in kidney and small intestine. Structural features include eight membrane-spanning alpha-helices and a unique loop-pore structure resembling ion channels.
Mice with homozygous deletions of the EAAC-1 transporter gene develop dicarboxylic aminoaciduria and display reduced spontaneous locomotor activity, although no neurodegeneration was observed over 12 months.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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