Thermo Fisher Scientific EAAC1 Monoclonal Antibody (N180/41), APC
EAAC1 단백질을 검출하기 위한 Thermo Fisher Scientific의 마우스 단클론 항체(APC 결합형). Western blot, ICC/IF, IP 등 다양한 응용 가능. 인간 및 랫트 반응성, 고순도 Protein G 정제, 액상 형태로 제공. 연구용으로만 사용.
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Thermo Fisher Scientific EAAC1 Monoclonal Antibody (N180/41), APC
Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:1,000 |
| Immunocytochemistry (ICC/IF) | 1:100 |
| Immunoprecipitation (IP) | Assay-dependent |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human, Rat |
| Host/Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | N180/41 |
| Immunogen | Fusion protein amino acids 1–524 (full length) of human EAAC1 |
| Conjugate | APC |
| Excitation/Emission Max | 651/660 nm |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein G |
| Storage Buffer | 95.64 mM phosphate / 2.48 mM MES, pH 7.4, with 0.5 M EDTA |
| Contains | No preservative |
| Storage Conditions | 4°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2932001 |
Additional Formats
- FITC (MA5-45548)
- PerCP (MA5-45549)
- Request custom conjugation
Product Specific Information
- 5 µg of MA5-45547 was sufficient for immunoprecipitation of EAAC1 from 50 µg of rat brain lysate.
- Detects approximately 60 kDa.
- This antibody was formerly sold as clone S180-41.
Target Information
L-glutamate is the major excitatory neurotransmitter in mammalian central nervous systems. To maintain glutamate levels below excitotoxic levels, excess glutamate at excitatory synaptic clefts is removed by ion-coupled glutamate transporters. Four glutamate transporters have been identified: GLAST-1, GLT-1, EAAC-1, and EAAT-4.
EAAC-1 is expressed in the CNS and also found in kidney and small intestine. Structural features include eight membrane-spanning alpha-helices and a loop-pore structure unique among secondary transporters. Mice with homozygous deletions of the EAAC-1 transporter gene develop dicarboxylic aminoaciduria and display reduced spontaneous locomotor activity without neurodegeneration over 12 months.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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