Thermo Fisher Scientific EAAC1 Monoclonal Antibody (N180/41), FITC
EAAC1 단백질을 검출하기 위한 FITC 결합 단일클론 항체로, WB, ICC/IF, IP 등 다양한 분석에 사용 가능. 인간 및 랫트 반응성, 단일클론 Mouse IgG1 형식. 498/517 nm 형광 특성으로 세포 내 EAAC1 시각화에 최적. 연구용 전용 제품.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:1,000 |
| Immunocytochemistry (ICC/IF) | 1:100 |
| Immunoprecipitation (IP) | Assay-dependent |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human, Rat |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | N180/41 |
| Immunogen | Fusion protein amino acids 1–524 (full length) of human EAAC1 |
| Conjugate | FITC |
| Excitation / Emission Max | 498 / 517 nm |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein G |
| Storage Buffer | 9.1 mM sodium bicarbonate/PBS, pH 7.4, with 640.91 mM DMSO, 136.36 mM ethanolamine |
| Contains | No preservative |
| Storage Conditions | 4°C, store in dark |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2932002 |
Additional Formats
- APC conjugate (MA5-45547)
- PerCP conjugate (MA5-45549)
- Custom conjugation available upon request
Product Specific Information
5 µg of MA5-45548 was sufficient for immunoprecipitation of EAAC1 from 50 µg of rat brain lysate. Detects approximately 60 kDa.
This antibody was formerly sold as clone S180-41.
Target Information
L-glutamate is the major excitatory neurotransmitter in mammalian central nervous systems. Excess glutamate is removed by ion-coupled glutamate transporters to prevent excitotoxicity. Four transporters have been identified: GLAST-1, GLT-1, EAAC-1, and EAAT-4.
EAAC-1 is expressed in the CNS, kidney, and small intestine. It features eight membrane-spanning alpha-helices and a unique loop-pore structure similar to ion channels, as well as a highly amphipathic helix forming a hydrophilic membrane pathway.
Mice with homozygous deletions of EAAC-1 show dicarboxylic aminoaciduria and reduced locomotor activity without neurodegeneration over 12 months.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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