Thermo Fisher Scientific H3K4me2 Recombinant Rabbit Monoclonal Antibody (RM135), ChIP-Verified
Histone H3K4me2에 특이적으로 반응하는 ChIP 검증된 재조합 Rabbit Monoclonal 항체. Western blot, ICC/IF, ELISA, ChIP, ChIP-Seq, Luminex 등 다양한 응용 가능. 교차 반응 없음, 고순도 Protein A 정제, -20°C 보관.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 0.02–0.2 µg/mL |
| Immunocytochemistry (ICC/IF) | 1–2 µg/mL |
| ELISA | 0.2–1 µg/mL |
| ChIP assay (ChIP) | 1–5 µg |
| ChIP-sequencing (ChIP-Seq) | 3 µg |
| Luminex (LUM) | 0.1–0.5 µg/mL |
Product Specifications
| Property | Description |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Rabbit / IgG |
| Expression System | HEK293 cells |
| Class | Recombinant Monoclonal |
| Type | Antibody |
| Clone | RM135 |
| Immunogen | A dimethyl-peptide corresponding to Dimethyl-Histone H3 (Lys4) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein A |
| Storage Buffer | PBS with 50% glycerol, 1% BSA |
| Contains | 0.09% sodium azide |
| Storage Conditions | -20°C |
| RRID | AB_2815521 |
Product Specific Information
This antibody specifically reacts with Histone H3 dimethylated at Lysine 4 (H3K4me2).
No cross-reactivity with H3K4me1, H3K4me3, or other histone H3 methylations.
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Target Information
Histone H3 is a DNA-binding protein found in chromatin of all eukaryotic cells. Together with four core histones, it forms the nucleosome structure.
Histones play key roles in transcription regulation, DNA repair, replication, and chromosomal stability.
Post-translational modifications of the H3 N-terminal tail, such as methylation, acetylation, and phosphorylation, influence chromatin structure and gene expression.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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