Thermo Fisher Scientific H3K4me3 Recombinant Rabbit Monoclonal Antibody (RM340), ChIP-Verified
Histone H3K4me3에 특이적으로 반응하는 재조합 토끼 단클론 항체로, ChIP 검증 완료. ELISA, ChIP, ChIP-Seq, Luminex 등 다양한 응용에 적합. 인간 시료 반응성, 고순도 Protein A 정제, 안정적 액상 형태로 제공.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| ELISA | 1–10 µg/mL |
| ChIP assay | 1–5 µg/mL |
| ChIP-sequencing | 3 µg |
| Luminex | 0.5–2 µg/mL |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Rabbit / IgG |
| Expression System | HEK293 cells |
| Class | Recombinant Monoclonal |
| Type | Antibody |
| Clone | RM340 |
| Immunogen | A trimethyl-peptide corresponding to Trimethyl-Histone H3 (Lys4) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein A |
| Storage Buffer | PBS with 50% glycerol, 1% BSA |
| Contains | 0.09% sodium azide |
| Storage Conditions | -20°C |
| RRID | AB_2866509 |
Product Specific Information
This antibody specifically reacts with Histone H3 trimethylated at Lysine 4 (H3K4me3).
No cross-reactivity with monomethylated (K4me1), dimethylated (K4me2), or other methylations in histone H3.
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Target Information
Histone H3 is one of the DNA-binding proteins found in the chromatin of all eukaryotic cells.
It, along with four core histone proteins, binds to DNA to form the nucleosome structure.
Histones play a central role in transcription regulation, DNA repair, DNA replication, and chromosomal stability.
Post-translational modifications of histones can alter chromatin structure or facilitate transcription factor binding.
The N-terminal tail of histone H3 undergoes methylation, acetylation, and phosphorylation, influencing cellular processes.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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(이미지 파일: MA5-33382-20UG_H3K4me3_P68431-1_Rabbit.svg, MA5-33382-20UG_H3K4me3_Q71DI3-1_Rabbit.svg 등)
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