
Thermo Fisher Scientific H3K4me2 Recombinant Rabbit Monoclonal Antibody (RM135), ChIP-Verified
Histone H3K4me2에 특이적으로 반응하는 재조합 토끼 단클론 항체로, ChIP 검증 완료. Western blot, ICC/IF, ELISA, ChIP, ChIP-Seq 등 다양한 응용 가능. HEK293 발현 시스템에서 제조되며 높은 특이성과 재현성을 제공. 연구용으로만 사용.
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Thermo Fisher Scientific H3K4me2 Recombinant Rabbit Monoclonal Antibody (RM135), ChIP-Verified
Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 0.02–0.2 µg/mL |
| Immunocytochemistry (ICC/IF) | 1–2 µg/mL |
| ELISA | 0.2–1 µg/mL |
| ChIP assay (ChIP) | 1–5 µg |
| ChIP-sequencing (ChIP-Seq) | 3 µg |
| Luminex (LUM) | 0.1–0.5 µg/mL |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Rabbit / IgG |
| Expression System | HEK293 cells |
| Class | Recombinant Monoclonal |
| Type | Antibody |
| Clone | RM135 |
| Immunogen | A dimethyl-peptide corresponding to Dimethyl-Histone H3 (Lys4) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein A |
| Storage Buffer | PBS with 50% glycerol, 1% BSA |
| Contains | 0.09% sodium azide |
| Storage Conditions | -20°C |
| RRID | AB_2866510 |
Product Specific Information
This antibody specifically reacts with Histone H3 dimethylated at Lysine 4 (K4me2).
No cross-reactivity with monomethylated (K4me1), trimethylated (K4me3), or other methylations in histone H3.
Click here for Master Lot linking information
Target Information
Histone H3 is a core DNA-binding protein within chromatin of eukaryotic cells. Together with other histones, it forms the nucleosome structure, playing key roles in:
- Transcription regulation
- DNA repair
- DNA replication
- Chromosomal stability
The N-terminal tail of histone H3 undergoes post-translational modifications such as methylation, acetylation, and phosphorylation, which influence chromatin structure and gene expression.
Usage Notes
For Research Use Only. Not for use in diagnostic procedures.
Not for resale without express authorization.
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