Thermo Fisher Scientific H3K9ac Recombinant Rabbit Monoclonal Antibody (RM161), ChIP-Verified
Histone H3의 Lys9 아세틸화(K9ac)에 특이적으로 반응하는 재조합 토끼 단클론 항체. ChIP 검증 완료. Western blot, IHC, ICC, ELISA, ChIP 등 다양한 응용 가능. 교차 반응 없음. 연구용으로만 사용.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 0.25–1 µg/mL |
| Immunohistochemistry (IHC) | 0.1–1 µg/mL |
| Immunocytochemistry (ICC/IF) | 0.5–2 µg/mL |
| ELISA | 0.5–2 µg/mL |
| ChIP assay (ChIP) | 2–10 µg/mL |
| ChIP-sequencing (ChIP-Seq) | 3 µg |
| Luminex (LUM) | 0.05–0.5 µg/mL |
Product Specifications
| Specification | Description |
|---|---|
| Host / Isotype | Rabbit / IgG |
| Expression System | HEK293 cells |
| Class | Recombinant Monoclonal |
| Type | Antibody |
| Clone | RM161 |
| Immunogen | An acetyl-peptide corresponding to Acetyl-Histone H3 (Lys9) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Storage Conditions | -20°C |
| Shipping Conditions | Dry ice |
| RRID | AB_2866511 |
Product Specific Information
This product reacts specifically with Histone H3 acetylated at Lysine 9 (H3K9ac).
No cross-reactivity with other acetylated lysines in histone H3.
Master Lot linking information (PDF)
Target Information
Histone H3 is one of the DNA-binding proteins found in the chromatin of all eukaryotic cells. Together with other core histones, it forms nucleosomes by binding DNA.
Histones play a central role in transcription regulation, DNA repair, replication, and chromosomal stability.
The N-terminal tail of histone H3 undergoes post-translational modifications such as methylation, acetylation, and phosphorylation, which influence chromatin structure and gene expression.
For Research Use Only.
Not for use in diagnostic procedures. Not for resale without express authorization.
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