Thermo Fisher Scientific H3K9ac Recombinant Rabbit Monoclonal Antibody (RM161), ChIP-Verified
ChIP 검증된 H3K9ac 특이적 recombinant rabbit monoclonal antibody로, Western blot, IHC, ICC, ELISA, ChIP, ChIP-Seq, Luminex 등 다양한 응용 가능. 인간 시료 반응성, HEK293 발현, Protein A 정제, -20°C 보관.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 0.25–1 µg/mL |
| Immunohistochemistry (IHC) | 0.1–1 µg/mL |
| Immunocytochemistry (ICC/IF) | 0.5–2 µg/mL |
| ELISA | 0.5–2 µg/mL |
| ChIP assay (ChIP) | 2–10 µg/mL |
| ChIP-sequencing (ChIP-Seq) | 3 µg |
| Luminex (LUM) | 0.05–0.5 µg/mL |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Rabbit / IgG |
| Expression System | HEK293 cells |
| Class | Recombinant Monoclonal |
| Type | Antibody |
| Clone | RM161 |
| Immunogen | An acetyl-peptide corresponding to Acetyl-Histone H3 (Lys9) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein A |
| Storage Buffer | PBS with 50% glycerol, 1% BSA |
| Contains | 0.09% sodium azide |
| Storage Conditions | -20°C |
| RRID | AB_2815522 |
Product Specific Information
This antibody reacts specifically with Histone H3 acetylated at Lysine 9 (H3K9ac) and shows no cross-reactivity with other acetylated lysines in histone H3.
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Target Information
Histone H3 is a DNA-binding protein found in the chromatin of all eukaryotic cells. Together with other core histone proteins, it forms the nucleosome structure that organizes DNA. Histones play essential roles in transcription regulation, DNA repair, replication, and chromosomal stability. Post-translational modifications such as methylation, acetylation, and phosphorylation on histone H3 tails influence chromatin structure and gene expression.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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