Thermo Fisher Scientific Caveolin 1 Polyclonal Antibody
Caveolin-1 단백질을 인식하는 Rabbit Polyclonal 항체로, WB, IHC, ICC 등 다양한 응용에 사용 가능. 합성 펩타이드 면역원 기반으로 높은 특이성과 재현성을 제공. 항원 친화 크로마토그래피로 정제되어 신뢰성 높은 결과 보장. 연구용으로만 사용.
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Applications and Tested Dilutions
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 1:1,000 | - |
| Immunohistochemistry (IHC) | - | View 1 publication |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:100 | - |
| Immunocytochemistry (ICC/IF) | 1:100 | - |
| Miscellaneous PubMed (Misc) | - | View 1 publication |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human |
| Published Species | Human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Synthetic peptide from N-terminus of human Caveolin-1 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS, pH 7.6, with 1% BSA |
| Contains | 0.1% sodium azide |
| Storage Conditions | Store at 4°C short term. For long term, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_1071036 |
Product Specific Information
Heat-mediated antigen retrieval is recommended prior to staining, using a 10 mM citrate buffer (pH 6.0) for 10 minutes, followed by cooling at room temperature for 20 minutes.
After antigen retrieval, incubate samples with primary antibody for 10 minutes at room temperature.
A suggested positive control is lung carcinoma tissue.
Target Information
Caveolin-1 (CAV1) is a scaffolding protein within caveolar membranes that interacts directly with G-protein alpha subunits to regulate their activity.
It is involved in co-stimulatory signaling essential for T-cell receptor (TCR)-mediated activation, and can bind with DPP4 to induce T-cell proliferation and NF-κB activation.
Mutations in the CAV1 gene are associated with congenital generalized lipodystrophy 3, pulmonary hypertension primary 3, and partial lipodystrophy/congenital cataracts with neurodegeneration syndrome.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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