Thermo Fisher Scientific CCK Polyclonal Antibody
Thermo Fisher Scientific의 CCK Polyclonal Antibody는 인간 CCK 단백질을 타깃으로 하는 Rabbit IgG 항체입니다. Western blot, IHC, ICC/IF 등 다양한 응용에 적합하며, 항원 친화 크로마토그래피로 정제되었습니다. PBS 버퍼에 1% BSA와 0.1% sodium azide가 포함되어 있습니다.
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Applications and Tested Dilutions
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | Assay-dependent | - |
| Immunohistochemistry (IHC) | - | 1 publication |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:200 | - |
| Immunocytochemistry (ICC/IF) | 10 µg/mL | - |
| In vitro Assay (IV) | - | 1 publication |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human |
| Published Species | Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Synthetic peptide within Human Cholecystokinin (CCK) aa 50 to the C-terminus (N-terminal) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS, pH 7.6, with 1% BSA |
| Contains | 0.1% sodium azide |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2072350 |
Product Specific Information
Heat-mediated antigen retrieval is recommended prior to staining, using a 10 mM citrate buffer (pH 6.0) for 10 minutes, followed by cooling at room temperature for 20 minutes.
After antigen retrieval, incubate samples with primary antibody for 10 minutes at room temperature.
A suggested positive control is stomach tissue.
Target Information
Cholecystokinin (CCK) is a neuropeptide hormone and neurotransmitter widely distributed throughout the gastrointestinal (GI) tract and central nervous system (CNS).
Antibodies that specifically react with CCK are useful for studying its mode of action, tissue expression, and localization in the CNS and peripheral neuroendocrine cells of the digestive system.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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