Thermo Fisher Scientific CD23 Polyclonal Antibody
CD23 단백질을 인식하는 Rabbit Polyclonal Antibody로, 인체 CD23(48–248aa) 재조합 단백질을 면역원으로 제작됨. 파라핀 절편 IHC에 적합하며, tonsil 조직을 양성 대조로 추천. 냉장(4°C) 단기 보관, 장기 보관 시 -20°C 권장. 연구용 전용 제품.
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Applications
Immunohistochemistry (Paraffin) (IHC (P))
- Tested Dilution: 1:100
- Suggested positive control: Tonsil tissue
Product Specifications
| 항목 | 내용 |
|---|---|
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Recombinant protein encoding human CD23 (48–248aa) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_2103175 |
Product Specific Information
Heat-mediated antigen retrieval is recommended prior to staining, using a 10 mM citrate buffer (pH 6.0) for 10 minutes followed by cooling at room temperature for 20 minutes.
After antigen retrieval, incubate samples with primary antibody for 10 minutes at room temperature.
A suggested positive control is tonsil tissue.
Target Information
CD23 is a 45 kDa glycoprotein present on a subpopulation of freshly isolated peripheral blood and tonsil B cells, and strongly expressed on EBV-transformed B lymphoblasts.
It is identical to the low-affinity IgE receptor found on B cells.
Expression of CD23 has been detected in neoplastic cells from cases of B cell chronic lymphocytic leukemia and some cases of centroblastic/centrocytic lymphoma.
Functionally, CD23 is involved in B cell growth, differentiation, and IgE production.
CD23 also has a soluble form that acts as a potent mitogenic factor.
Diseases associated with CD23 dysfunction include chronic conjunctivitis and chronic lymphocytic leukemia.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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