Thermo Fisher Scientific beta Catenin Polyclonal Antibody
Beta-catenin 단백질을 인식하는 Thermo Fisher Scientific의 rabbit polyclonal antibody. Western blot, IHC, ICC/IF 등 다양한 응용 가능. 세포 부착 및 신호전달 연구에 적합. 장기 보관 시 -20°C에서 안정적 유지.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:1,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:250 |
| Immunohistochemistry (Frozen) (IHC (F)) | 1:200 |
| Immunocytochemistry (ICC/IF) | 1:100 |
| Immunoprecipitation (IP) | 5 µg/mL |
Product Specifications
| Specification | Description |
|---|---|
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Synthetic peptide from the C-terminus of the catenin-beta protein |
| Conjugate | Unconjugated |
| Form | Liquid |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2088223 |
Product Specific Information
This antibody is predicted to react with bovine, amphibian, mouse, and rat based on sequence homology.
Heat-mediated antigen retrieval is recommended prior to staining, using a 10 mM citrate buffer (pH 6.0) for 10 minutes, followed by cooling at room temperature for 20 minutes.
After antigen retrieval, incubate samples with primary antibody for 10 minutes at room temperature.
A suggested positive control is breast carcinoma tissue.
Target Information
Beta-catenin is an adherens junction (AJ) protein involved in cell-cell adhesion and signaling.
It interacts with E-cadherin and alpha-catenin, linking the adhesion complex to the actin cytoskeleton.
Beta-catenin also associates with the adenomatous polyposis coli protein and microtubules, participating in Wnt/Wingless signaling pathways.
Its nuclear function includes co-activation of Lef/Tcf transcription factors, promoting transcription of genes such as cyclin D and c-myc that drive cell proliferation.
Aberrant beta-catenin activity is implicated in various cancers including colorectal, breast, liver, lung, ovarian, and skin tumors.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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