![Thermo Fisher Scientific CD34 Monoclonal Antibody (4H11[APG]), PE](https://images.cacheby.com/product-images/cacheby/57056430-28b8-11f0-bf4f-b7c32b12ae43/primary-antibody-image.jpg-650.jpg)
Thermo Fisher Scientific CD34 Monoclonal Antibody (4H11[APG]), PE
CD34 단백질을 인식하는 마우스 단일클론 항체로, PE 형광표지되어 세포분석 및 줄기세포 연구에 적합합니다. 인간 시료에 반응하며, 유세포분석(Flow Cytometry) 및 면역세포염색(ICC/IF)에 사용 가능합니다. 4°C 암소 보관, 동결 금지.
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Applications
Immunocytochemistry (ICC/IF)
- Assay-dependent
Flow Cytometry (Flow)
- 20 µL x 100 µL whole blood or 10⁶ cells
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Published Species | Human |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 4H11 [APG] |
| Immunogen | Permanent human cell line from a patient with myeloid leukaemia |
| Conjugate | PE (R-Phycoerythrin) |
| Excitation / Emission Max | 565 / 576 nm |
| Form | Liquid |
| Purification | Size-exclusion chromatography |
| Storage Buffer | PBS, pH 7.4, with 0.2% BSA |
| Contains | 15 mM sodium azide |
| Storage Conditions | 4°C, store in dark, DO NOT FREEZE |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_1072204 |
Additional Formats
- Unconjugated (MA1-19229)
- Alexa Fluor 488 (MA5-18136)
- APC (MA1-19466)
- Biotin (MA1-19515)
- FITC (MA1-19593)
- Custom conjugation available upon request
Product Specific Information
This antibody reacts with extracellular class III epitope on CD34, a 110–115 kDa monomeric transmembrane phosphoglycoprotein expressed on hematopoietic progenitor cells and pluripotential stem cells. Expression is gradually lost on progenitor cells. The antibody 4H11 [APG] completely blocks binding of class III antibodies BIRMA K3 and 8G12 on the KG1a cell line.
Target Information
CD34 is a highly glycosylated monomeric surface protein (111–115 kDa) present on many stem cell populations. It serves as a stem cell marker, with reversible expression on human hematopoietic stem cells. CD34 may function in receptor-mediated endocytosis, adhesion, differentiation, and proliferation of hematopoietic progenitors. It likely mediates attachment of stem cells to bone marrow extracellular matrix or stromal cells and may act as a scaffold for glycan attachment, aiding in selectin binding. The intracellular domain can be phosphorylated by protein kinase C, suggesting a signaling role. CD34 dysfunction is associated with dermatofibrosarcoma and neurofibroma.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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