Thermo Fisher Scientific CD27 Monoclonal Antibody (LT27), PE

Applications

• Flow Cytometry (Flow)

Tested Dilution

• 20 µL per 100 µL whole blood or 10⁶ cells

Product Specifications

Additional Formats

• Unconjugated
• APC
• FITC
• Request custom conjugation

Product Specific Information

This antibody reacts with an extracellular epitope of CD27 (T14), a 50–55 kDa type I transmembrane glycoprotein (member of the TNF-receptor superfamily) expressed on medullary thymocytes, peripheral T lymphocytes, some B lymphocytes, and NK cells.

Target Information

CD27 is a 50 kDa member of the tumor necrosis factor (TNF) receptor superfamily that includes CD40 and CD30. TNF superfamily members regulate cell proliferation and death. Expression of CD27 and its ligand CD70 is mainly confined to lymphocytes. High CD27 expression depends on antigen receptor ligation, while CD70 requires co-stimulatory or pro-inflammatory signals.

CD27 is expressed as a disulfide-linked homodimer on mature thymocytes, peripheral blood T cells, and a subpopulation of B cells. Activation of T cells via TCR-CD3 complex upregulates CD27 expression and releases soluble CD27 (sCD27, 28–32 kDa) into plasma, urine, or spinal fluid. Soluble CD27 serves as a prognostic marker for acute and chronic B cell malignancies.

RgpA, a cysteine proteinase, degrades CD27 and counteracts T cell activation mediated by CD27 and CD70. CD27-binding protein (SIVA) is a proapoptotic protein that interacts with CD27 and may play a role in apoptosis induced by this receptor. Diseases associated with CD27 dysfunction include Lymphoproliferative Syndrome 2 and Autosomal Recessive Lymphoproliferative Syndrome.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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