Thermo Fisher Scientific CD107a (LAMP-1) Monoclonal Antibody (eBioH4A3), eFluor 450, eBioscience
CD107a(LAMP-1)을 인식하는 eBioH4A3 단클론 항체로, eFluor 450 형광체 결합. Flow cytometry 및 intracellular staining에 최적화됨. 인간 시료에서 검증됨. 405/445 nm(Violet laser)에서 발광. 4°C 보관, 동결 금지.
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카탈로그
2개 옵션 · 카탈로그 번호를 클릭하면 복사됩니다Thermo Fisher Scientific · Thermo Fisher Scientific CD107a (LAMP-1) Monoclonal Antibody (eBioH4A3), eFluor 450, eBioscience
Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Immunohistochemistry (IHC) | - | View 1 publication |
| Immunocytochemistry (ICC/IF) | - | View 7 publications |
| Flow Cytometry (Flow) | 5 µL (0.25 µg)/test | View 9 publications |
Product Specifications
| 항목 | 내용 |
|---|---|
| Published species | Human |
| Host / Isotype | Mouse / IgG1, kappa |
| Recommended Isotype Control | Mouse IgG1 kappa Isotype Control (P3.6.2.8.1), eFluor™ 450, eBioscience™ |
| Class | Monoclonal |
| Type | Antibody |
| Clone | eBioH4A3 |
| Conjugate | eFluor™ 450 |
| Excitation/Emission Max | 405/445 nm |
| Form | Liquid |
| Concentration | 5 µL/Test |
| Storage conditions | 4°C, store in dark, DO NOT FREEZE |
| Shipping conditions | Wet ice |
| RRID | AB_2574034 |
Available Formats
- Unconjugated
- Alexa Fluor 488
- Brilliant Violet 421
- Brilliant Violet 650
- Brilliant Violet 711
- eFluor 660
- FITC
- PE
- PE-Cyanine5
- PE-Cyanine7
- PE-eFluor 610
- PerCP-eFluor 710
- Request custom conjugation
Product Specific Information
The eBioH4A3 monoclonal antibody reacts with human CD107a (LAMP-1), a highly glycosylated ~110 kDa protein expressed mainly on lysosomal/endosomal membranes. CD107a transiently appears on the surface of degranulating cytolytic T cells, activated platelets, and some cancer cells.
Applications Reported:
Suitable for intracellular staining and flow cytometric analysis.
Applications Tested:
Pre-titrated and validated for intracellular staining and flow cytometric analysis of Jurkat cells using the Intracellular Fixation & Permeabilization Buffer Set (Product #88-8824-00).
Recommended use: 5 µL (0.25 µg) per test (100 µL total volume, 10⁵–10⁸ cells/test).
Fluorochrome Information:
eFluor® 450 is an alternative to Pacific Blue®, excited at 405 nm and emitting at 445 nm. Ensure instrument compatibility with the Violet laser.
Filtration:
0.2 µm post-manufacturing filtered.
Target Information
LAMP1 (CD107a) is a major lysosomal membrane component, with 1–2% also found on the plasma membrane. It is a heavily glycosylated type I membrane protein involved in lysosome biogenesis and fusion with phagosomes. LAMP1 relocation to the plasma membrane depends on a carboxyl-terminal tyrosine-based motif (YXXI). Mutations in this motif disrupt lysosomal localization.
Cell surface LAMP1 promotes adhesion of PBMCs to vascular endothelium, suggesting a role in inflammatory cell recruitment. Increased LAMP1 expression is observed in neurons and glial cells near senile plaques in Alzheimer's disease and in activated immune cells during thymic involution. LAMP1 serves as a reliable marker of mast cell activation.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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