Thermo Fisher Scientific CD105 (Endoglin) Monoclonal Antibody (MJ7/18), eFluor 450, eBioscience

Applications

Tested Dilution

Publications

Immunohistochemistry (IHC)

-

View 5 publications 5 publications

Immunohistochemistry (Frozen) (IHC (F))

-

View 2 publications 2 publications

Immunocytochemistry (ICC/IF)

-

View 2 publications 2 publications

Flow Cytometry (Flow)

1 µg/test

View 22 publications 22 publications

ELISA (ELISA)

-

View 1 publication 1 publication

Product Specifications

Published species

Mouse

Host/Isotype

Rat / IgG2a, kappa

Recommended Isotype Control

Rat IgG2a kappa Isotype Control (eBR2a), eFluor™ 450, eBioscience™

Class

Monoclonal

Type

Antibody

Clone

MJ7/18

Conjugate

eFluor™ 450 eFluor™ 450 eFluor™ 450

View additional formats

• Unconjugated
• Alexa Fluor 488
• APC
• Biotin
• PE
• PE-Cyanine7
• PerCP-eFluor 710
• Request custom conjugation

Excitation/Emission Max

405/445 nm View spectra

Form

Liquid

Concentration

0.2 mg/mL

Storage conditions

4° C, store in dark, DO NOT FREEZE!

Shipping conditions

Wet ice

RRID

AB_10547279

Product Specific Information

Description: The MJ7/18 monoclonal antibody reacts with the mouse CD105 molecule, also known as Endoglin. This 90 kDa disulfide-linked homodimer is expressed by vascular endothelial cells. It is suggested that CD105 functions in adhesion and embryonic angiogenesis.

Applications Reported: This MJ7/18 antibody has been reported for use in flow cytometric analysis.

Applications Tested: This MJ7/18 antibody has been tested by flow cytometric analysis of bEnd.3 cells. This can be used at less than or equal to 1 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

eFluor® 450 is an alternative to Pacific Blue®. eFluor® 450 emits at 445 nm and is excited with the Violet laser (405 nm). Please make sure that your instrument is capable of detecting this fluorochome.

Excitation: 405 nm; Emission: 445 nm; Laser: Violet Laser.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

CD105 or Endoglin is a Type I transmembrane protein, which is highly expressed on human vascular endothelial cells. It exists on an O- and N-glycosylated homodimer. Up regulation of endoglin expression has been demonstrated in tumor vasculature and proliferating cells, suggesting that it is a proliferation associated endothelial marker. CD105 binds to TGF beta 1 and 3 with high affinity but not to TGF beta 2.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.