Thermo Fisher Scientific PLK1 Polyclonal Antibody
인간 PLK1 단백질을 인식하는 닭 유래 폴리클로날 항체입니다. Western blot, ICC/IF, ELISA에 사용 가능합니다. 세포 분열 및 암 발생 연구에 유용하며, -20°C에서 보관 시 안정적입니다. 연구용으로만 사용됩니다.
- 카탈로그번호
- 200-901-MH7x (2개 옵션)
- 판매단위
- pk
카탈로그
2개 옵션 · 카탈로그 번호를 클릭하면 복사됩니다Thermo Fisher Scientific · Thermo Fisher Scientific PLK1 Polyclonal Antibody
Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500 |
| Immunocytochemistry (ICC/IF) | 5 µg/mL |
| ELISA | 1:10,000–1:50,000 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Chicken / IgY |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Synthetic peptide corresponding to an internal portion of human PLK1 conjugated to Keyhole Limpet Hemocyanin (KLH) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1.05 mg/mL |
| Purification | Selective precipitation |
| Storage Buffer | PBS, pH 7 |
| Contains | 0.01% sodium azide |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
Store vial at -20°C prior to opening. Aliquot contents and freeze at -20°C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use.
This product is an IgY fraction antibody purified from monospecific chicken egg yolks by a multi-step process including selective precipitation and salt fractionation followed by extensive dialysis against PBS buffer. Reactivity occurs against human PLK1. BLAST analysis suggests cross-reactivity with PLK1 from rat, mouse, and bovine sources based on 100% sequence homology.
Target Information
PLK1 (Polo-like kinase 1) is a serine/threonine kinase involved in cell mitosis and is a marker of cell proliferation. It plays a critical role in carcinogenesis. Polo-like kinases act by binding and phosphorylating proteins already phosphorylated on specific motifs recognized by the POLO box domains. PLK1 phosphorylates multiple proteins including BORA, BUB1B/BUBR1, CCNB1, CDC25C, CEP55, ECT2, ERCC6L, FBXO5/EMI1, FOXM1, KIF20A/MKLP2, CENPU, NEDD1, NINL, NPM1, NUDC, PKMYT1/MYT1, KIZ, PPP1R12A/MYPT1, PRC1, RACGAP1/CYK4, SGOL1, STAG2/SA2, TEX14, TOPORS, TP73, TPT1, and WEE1. It is essential for centrosome function and assembly of bipolar spindles.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Thermo Fisher Scientific 상품 둘러보기
전체보기
문의
0개 · 배송·재고 문의는 실시간 상담이 빠릅니다아직 등록된 문의가 없어요.