Thermo Fisher Scientific PLK1 Polyclonal Antibody

Applications and Tested Dilution

Product Specifications

Product Specific Information

Store vial at -20°C prior to opening. Aliquot contents and freeze at -20°C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use.

This product is an IgY fraction antibody purified from monospecific chicken egg yolks by a multi-step process including selective precipitation and salt fractionation followed by extensive dialysis against PBS buffer. Reactivity occurs against human PLK1. BLAST analysis suggests cross-reactivity with PLK1 from rat, mouse, and bovine sources based on 100% sequence homology.

Target Information

PLK1 (Polo-like kinase 1) is a serine/threonine kinase involved in cell mitosis and is a marker of cell proliferation. It plays a critical role in carcinogenesis. Polo-like kinases act by binding and phosphorylating proteins already phosphorylated on specific motifs recognized by the POLO box domains. PLK1 phosphorylates multiple proteins including BORA, BUB1B/BUBR1, CCNB1, CDC25C, CEP55, ECT2, ERCC6L, FBXO5/EMI1, FOXM1, KIF20A/MKLP2, CENPU, NEDD1, NINL, NPM1, NUDC, PKMYT1/MYT1, KIZ, PPP1R12A/MYPT1, PRC1, RACGAP1/CYK4, SGOL1, STAG2/SA2, TEX14, TOPORS, TP73, TPT1, and WEE1. It is essential for centrosome function and assembly of bipolar spindles.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.