Thermo Fisher Scientific XRCC1 Polyclonal Antibody
XRCC1 단백질을 인식하는 Thermo Fisher Scientific의 다클론 항체로, 인간 및 생쥐 시료에 반응합니다. Western blot, ICC/IF, ELISA 등에 적합하며 IgY 타입으로 고순도 정제된 액상 형태입니다. DNA 수선 관련 연구용으로 최적화된 제품입니다.
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Applications
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:1,000 |
| Immunocytochemistry (ICC/IF) | 10 µg/mL |
| ELISA | 1:10,000–1:50,000 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse |
| Host / Isotype | Chicken / IgY |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Anti-XRCC1 antibody was prepared from eggs of chickens laid after repeated immunizations with a synthetic peptide corresponding to a N-terminal portion of human XRCC1 conjugated to Keyhole Limpet Hemocyanin (KLH). |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.93 mg/mL |
| Purification | Selective precipitation |
| Storage Buffer | PBS, pH 7 |
| Contains | 0.01% sodium azide |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
Store vial at -20°C prior to opening. Aliquot contents and freeze at -20°C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use.
This purified antibody is directed against human XRCC1. It is an IgY fraction antibody purified from monospecific chicken egg yolks by a multi-step process including selective precipitation and salt fractionation followed by extensive dialysis against the buffer stated above.
Target Information
XRCC1 (X-ray cross complementing factor-1) is involved in DNA base excision repair. XRCC1 is a 70 kDa protein that associates with other DNA repair enzymes including poly (ADP-ribose) polymerase (PARP), DNA Ligase III, and DNA polymerase beta. XRCC1 contains a BRCT domain (BRCA1 carboxyl terminus) which mediates protein-protein interaction with DNA ligase III-alpha.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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