Thermo Fisher Scientific XRCC1 Polyclonal Antibody
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Applications
Tested Dilution
Publications
Western Blot (WB)
1:500-1:1,000
Immunocytochemistry (ICC/IF)
10 µg/mL
ELISA (ELISA)
1:10,000-1:50,000
Product Specifications
Species Reactivity
Human, Mouse
Host/Isotype
Chicken / IgY
Class
Polyclonal
Type
Antibody
Immunogen
Anti-XRCC1 antibody was prepared from eggs of chickens laid after repeated immunizations with a synthetic peptide corresponding to a N-Terminal portion of human XRCC1 conjugated to Keyhole Limpet Hemocyanin (KLH). if (typeof window.$mangular === undefined
|| !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{
targetFamily:
XRCC1,
uniProtId:
P18887-1,
ncbiNodeId:
9606,
antigenRange:
1,
antigenLength:
633,
antigenImageFileName:
200-901-MG5_XRCC1_P18887-1_Chicken.svg,
antigenImageFileNamePDP:
200-901-MG5_XRCC1_P18887-1_Chicken_PDP.jpeg,
sortOrder:
1}\]
; $mangular.isB2BCMGT = false
; $mangular.isEpitopesModalImageMultiSizeEnabled = true
;
View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}
Conjugate
Unconjugated Unconjugated Unconjugated
Form
Liquid
Concentration
0.93 mg/mL
Purification
selective precipitation
Storage buffer
PBS, pH 7
Contains
0.01% sodium azide
Storage conditions
-20° C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
Product Specific Information
Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
This purified antibody is directed against human XRCC1. This product is an IgY fraction antibody purified from monospecific chicken egg yolks by a multi-step process which includes selective precipitation and salt fractionation followed by extensive dialysis against the buffer stated above.
Target Information
XRCC1 (X-ray cross complementing factor-1) is involved in DNA base excision repair. XRCC1 is a 70 kDa protein that has been shown to be physically associated with other DNA repair enzymes, including poly (ADP-ribose) polymerase (PARP), DNA Ligase III and DNA polymerase beta. XRCC1 contains a BRCT domain (for BRCA1 carboxyl terminus) which was originally found in the tumor suppressor protein BRCA1. Recently, the BRCT domain has been shown to mediate the protein-protein interaction of XRCC1 and DNA ligase III-alpha.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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