Thermo Fisher Scientific EIF4E Polyclonal Antibody

Applications

Tested Dilution

Publications

Western Blot (WB)

5 µg/mL

-

Immunohistochemistry (IHC)

1:1,000

-

ELISA (ELISA)

Assay-dependent

-

Product Specifications

Species Reactivity

Human, Mouse

Host/Isotype

Chicken / IgY

Class

Polyclonal

Type

Antibody

Immunogen

Anti-EIF4E antibody was prepared from eggs of chickens laid after repeated immunizations with a synthetic peptide corresponding to a near C-Terminal portion of human EIF4E conjugated to Keyhole Limpet Hemocyanin (KLH). if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:eIF4E,uniProtId:P06730-1,ncbiNodeId:9606,antigenRange:217,antigenLength:217,antigenImageFileName:200-901-MK9_eIF4E_P06730-1_Chicken.svg,antigenImageFileNamePDP:200-901-MK9_eIF4E_P06730-1_Chicken_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

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Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1.05 mg/mL

Purification

selective precipitation

Storage buffer

PBS, pH 7

Contains

0.01% sodium azide

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Ambient (domestic); Wet ice (international)

Product Specific Information

Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

This affinity purified antibody is directed against human EIF4E. This product is an IgY fraction antibody purified from monospecific chicken egg yolks by a multi-step process which includes selective precipitation and salt fractionation followed by extensive dialysis against the buffer stated above.A BLAST analysis was used to suggest cross-reactivity with the antigen based on 100% homology with the immunizing sequence to bovine and 93% homology to rat, mouse, and rabbit.

Target Information

eIF4E is involved in several cellular processes including enhanced translational efficiency, splicing, mRNA stability, and RNA nuclear export. All eukaryotic cellular mRNAs are blocked at their 5-prime ends with the 7-methylguanosine cap structure, m7GpppX (where X is any nucleotide). EIF4E is a eukaryotic translation initiation factor involved in directing ribosomes to the cap structure of mRNAs. It is a 24-kD polypeptide that exists as both a free form and as part of a multiprotein complex termed EIF4F. The EIF4E polypeptide is the rate-limiting component of the eukaryotic translation apparatus and is involved in the mRNA-ribosome binding step of eukaryotic protein synthesis. The other subunits of EIF4F are a 50-kD polypeptide, termed EIF4A, that possesses ATPase and RNA helicase activities, and a 220-kD polypeptide, EIF4G.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.