Thermo Fisher Scientific EIF4E Polyclonal Antibody
Human 및 Mouse 반응성을 가지는 Chicken 유래 Polyclonal IgY 항체로 EIF4E 단백질을 검출. Western blot, IHC, ELISA 등 다양한 응용 가능. 고순도 액상 형태로 제공되며 -20°C에서 안정적 보관 가능.
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Applications
| Application | Tested Dilution | Notes |
|---|---|---|
| Western Blot (WB) | 5 µg/mL | |
| Immunohistochemistry (IHC) | 1:1,000 | |
| ELISA | Assay-dependent |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human, Mouse |
| Host / Isotype | Chicken / IgY |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Anti-EIF4E antibody was prepared from eggs of chickens laid after repeated immunizations with a synthetic peptide corresponding to a near C-terminal portion of human EIF4E conjugated to Keyhole Limpet Hemocyanin (KLH). |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1.05 mg/mL |
| Purification | Selective precipitation |
| Storage Buffer | PBS, pH 7 |
| Contains | 0.01% sodium azide |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
Store vial at -20°C prior to opening. Aliquot contents and freeze at -20°C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature.
This product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use.
This affinity purified antibody is directed against human EIF4E. It is an IgY fraction antibody purified from monospecific chicken egg yolks by a multi-step process including selective precipitation and salt fractionation followed by extensive dialysis against the buffer stated above.
BLAST analysis suggests cross-reactivity with bovine (100% homology) and partial homology (93%) to rat, mouse, and rabbit.
Target Information
eIF4E is involved in several cellular processes including enhanced translational efficiency, splicing, mRNA stability, and RNA nuclear export.
All eukaryotic cellular mRNAs are capped at their 5′ ends with the 7-methylguanosine structure (m7GpppX). EIF4E directs ribosomes to this cap structure and acts as a rate-limiting component in eukaryotic translation initiation.
EIF4E exists as a free form and as part of the multiprotein complex EIF4F, which also includes EIF4A (ATPase/RNA helicase) and EIF4G (scaffold protein).
For Research Use Only. Not for use in diagnostic procedures or resale without authorization.
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